PMID- 25176081
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20181202
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 34
IP  - 8
DP  - 2014 Jul
TI  - [Establishment of a rat model of acute liver failure by a modified 90% bloodless 
      hepatectomy and by D-galactosamine and lipopolysaccharide injection].
PG  - 1135-9
AB  - OBJECTIVE: To compare the effects of different approaches to establishing rat 
      models of acute liver failure (ALF). METHODS: Sixty-eight Sprague-Dawley rats 
      were randomly divided into 3 groups for establishing ALF models using 3 different 
      approaches, namely conventional hepatectomy for resecting 90% liver tissue as 
      described by Higgins and Anderson, modified bloodless hepatectomy for resecting 
      90% liver tissue, and intraperitoneal injections of 700 mg/kg D-galactosamine 
      (D-gal) and 5 microg/kg lipopolysaccharide (LPS). The mortality of the rats due to 
      postoperative bleeding and survival rate at 7 days after the surgery were 
      recorded. The levels of alanine aminotransferase (ALT), total bilimbin (Tbil), 
      albumin (ALB), NH3, glucose (Glu) and prothrombin time (PT) were monitored, and 
      histopathologies of the liver were examined at 24 and 72 h after the surgery. 
      RESULTS: The mortality rate due to postoperative bleeding was higher in 
      conventional hepatectomy group than in the modified surgical group (15% vs 0). 
      The survival rate at 7 days was 25%, 0%, 15% in conventional surgical group, 
      modified surgical group and drug injection group, respectively. In the latter two 
      groups, significant changes of ALT, Tbil, ALB, NH3, Glu, and PT were recorded at 
      24 and 72 h after the modeling (P<0.05), and these changes were the most obvious 
      at 24 h in modified surgical group and at 72 h in the drug injection group; ALB 
      in both groups declined to the lowest at 7 days and then increased gradually. 
      Liver cell degeneration and necrosis were found in modified surgical group and 
      drug injection group at 24 h and 72 h after the modeling. CONCLUSION: Both the 
      modified 90% bloodless hepatectomy and injections of D-gal and LPS can be used to 
      establish ideal rat models of ALF to suit different ALF-related researches.
FAU - Gong, Xumeng
AU  - Gong X
AD  - Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical 
      University, Guangzhou 510282, China.E-mail: meng623532018@163.com.
FAU - Zhou, Bin
AU  - Zhou B
FAU - Chen, Huamu
AU  - Chen H
FAU - Yang, Fangyuan
AU  - Yang F
FAU - Huang, Yuezhao
AU  - Huang Y
FAU - Zhong, Jisheng
AU  - Zhong J
FAU - Gao, Yi
AU  - Gao Y
LA  - chi
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Lipopolysaccharides)
RN  - 7535-00-4 (Galactosamine)
SB  - IM
MH  - Animals
MH  - *Disease Models, Animal
MH  - Galactosamine/adverse effects
MH  - Hepatectomy
MH  - Injections, Intraperitoneal
MH  - Lipopolysaccharides/adverse effects
MH  - Liver Failure, Acute/chemically induced/*pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2014/09/02 06:00
MHDA- 2015/09/02 06:00
CRDT- 2014/09/02 06:00
PHST- 2014/09/02 06:00 [entrez]
PHST- 2014/09/02 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2014 Jul;34(8):1135-9.