PMID- 25180967
OWN - NLM
STAT- MEDLINE
DCOM- 20150826
LR  - 20220129
IS  - 1751-7370 (Electronic)
IS  - 1751-7362 (Print)
IS  - 1751-7362 (Linking)
VI  - 9
IP  - 3
DP  - 2015 Mar
TI  - Nitrotoga-like bacteria are previously unrecognized key nitrite oxidizers in 
      full-scale wastewater treatment plants.
PG  - 708-20
LID - 10.1038/ismej.2014.158 [doi]
AB  - Numerous past studies have shown members of the genus Nitrospira to be the 
      predominant nitrite-oxidizing bacteria (NOB) in nitrifying wastewater treatment 
      plants (WWTPs). Only recently, the novel NOB 'Candidatus Nitrotoga arctica' was 
      identified in permafrost soil and a close relative was enriched from activated 
      sludge. Still, little is known about diversity, distribution and functional 
      importance of Nitrotoga in natural and engineered ecosystems. Here we developed 
      Nitrotoga 16S rRNA-specific PCR primers and fluorescence in situ hybridization 
      (FISH) probes, which were applied to screen activated sludge samples from 20 
      full-scale WWTPs. Nitrotoga-like bacteria were detected by PCR in 11 samples and 
      reached abundances detectable by FISH in seven sludges. They coexisted with 
      Nitrospira in most of these WWTPs, but constituted the only detectable NOB in two 
      systems. Quantitative FISH revealed that Nitrotoga accounted for nearly 2% of the 
      total bacterial community in one of these plants, a number comparable to 
      Nitrospira abundances in other WWTPs. Spatial statistics revealed that Nitrotoga 
      coaggregated with ammonia-oxidizing bacteria, strongly supporting a functional 
      role in nitrite oxidation. This activity was confirmed by FISH in combination 
      with microradiography, which revealed nitrite-dependent autotrophic carbon 
      fixation by Nitrotoga in situ. Correlation of the presence or absence with WWTP 
      operational parameters indicated low temperatures as a main factor supporting 
      high Nitrotoga abundances, although in incubation experiments these NOB remained 
      active over an unexpected range of temperatures, and also at different ambient 
      nitrite concentrations. In conclusion, this study demonstrates that Nitrotoga can 
      be functionally important nitrite oxidizers in WWTPs and can even represent the 
      only known NOB in engineered systems.
FAU - Lucker, Sebastian
AU  - Lucker S
AD  - Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, 
      University of Vienna, Vienna, Austria.
FAU - Schwarz, Jasmin
AU  - Schwarz J
AD  - Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, 
      University of Vienna, Vienna, Austria.
FAU - Gruber-Dorninger, Christiane
AU  - Gruber-Dorninger C
AD  - Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, 
      University of Vienna, Vienna, Austria.
FAU - Spieck, Eva
AU  - Spieck E
AD  - University of Hamburg, Biocenter Klein Flottbek, Microbiology and Biotechnology, 
      Hamburg, Germany.
FAU - Wagner, Michael
AU  - Wagner M
AD  - Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, 
      University of Vienna, Vienna, Austria.
FAU - Daims, Holger
AU  - Daims H
AD  - Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, 
      University of Vienna, Vienna, Austria.
LA  - eng
SI  - GENBANK/KM017111
SI  - GENBANK/KM017112
SI  - GENBANK/KM017113
SI  - GENBANK/KM017114
SI  - GENBANK/KM017115
SI  - GENBANK/KM017116
SI  - GENBANK/KM017117
SI  - GENBANK/KM017118
SI  - GENBANK/KM017119
SI  - GENBANK/KM017120
SI  - GENBANK/KM017121
SI  - GENBANK/KM017122
SI  - GENBANK/KM017123
SI  - GENBANK/KM017124
SI  - GENBANK/KM017125
SI  - GENBANK/KM017126
SI  - GENBANK/KM017127
SI  - GENBANK/KM017128
SI  - GENBANK/KM017129
GR  - P 24101/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140902
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - 0 (DNA Primers)
RN  - 0 (Nitrites)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia/metabolism
MH  - Chemoautotrophic Growth
MH  - DNA Primers
MH  - Gallionellaceae/classification/isolation & purification/*metabolism
MH  - Molecular Sequence Data
MH  - Nitrites/*metabolism
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/*microbiology
MH  - *Waste Disposal Facilities
PMC - PMC4270731
MID - EMS59699
OID - NLM: EMS59699
EDAT- 2014/09/03 06:00
MHDA- 2015/08/27 06:00
PMCR- 2016/03/01
CRDT- 2014/09/03 06:00
PHST- 2014/03/31 00:00 [received]
PHST- 2014/07/04 00:00 [revised]
PHST- 2014/07/18 00:00 [accepted]
PHST- 2014/09/03 06:00 [entrez]
PHST- 2014/09/03 06:00 [pubmed]
PHST- 2015/08/27 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - ismej2014158 [pii]
AID - 10.1038/ismej.2014.158 [doi]
PST - ppublish
SO  - ISME J. 2015 Mar;9(3):708-20. doi: 10.1038/ismej.2014.158. Epub 2014 Sep 2.