PMID- 25182355
OWN - NLM
STAT- MEDLINE
DCOM- 20150514
LR  - 20160511
IS  - 2042-6984 (Electronic)
IS  - 2042-6976 (Linking)
VI  - 4 Suppl 2
DP  - 2014 Sep
TI  - In vitro allergy testing.
PG  - S46-50
LID - 10.1002/alr.21384 [doi]
AB  - BACKGROUND: History and physical examination are the first, and most important 
      steps, in the evaluation of a patient suspected of having an allergy. The 
      diagnosis can be confirmed with either skin or serum testing for evidence of 
      immunoglobulin E (IgE)-mediated reactivity. METHODS: The recent literature on 
      serum-based testing for the detection and quantitation of allergen specific IgE 
      (sIgE) was reviewed, identifying where available the "best practices" from high 
      level of evidence studies and/or physician organization guidelines. RESULTS: 
      Current practices for documenting sIgE are detailed, including enzyme-linked 
      immunoassays on conventional extracts (standardized or not), similar on 
      microarrays of highly purified or recombinant allergens, and basophil activation 
      testing. CONCLUSION: Serum testing is an equal alternative to skin testing for 
      establishing the presence of IgE-mediated sensitivity and for identifying the 
      allergens involved. Like skin testing, limitations include the availability of 
      fully detailed allergenic extracts, particularly for foods, drugs, and 
      occupational agents, and the possibility of non-IgE mediated issues.
CI  - (c) 2014 ARS-AAOA, LLC.
FAU - Osguthorpe, John David
AU  - Osguthorpe JD
AD  - Department of Surgery, Veteran's Administration Hospital, Charleston, SC.
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Int Forum Allergy Rhinol
JT  - International forum of allergy & rhinology
JID - 101550261
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Basophils/immunology
MH  - Humans
MH  - Hypersensitivity/blood/*diagnosis/immunology
MH  - Immunoglobulin E/blood
MH  - Immunologic Tests
OTO - NOTNLM
OT  - allergy
OT  - hypersensitivity, immediate
OT  - immunologic tests
OT  - in vitro
OT  - nasal provocation tests
OT  - skin tests
EDAT- 2014/09/04 06:00
MHDA- 2015/05/15 06:00
CRDT- 2014/09/04 06:00
PHST- 2014/06/13 00:00 [received]
PHST- 2014/06/30 00:00 [accepted]
PHST- 2014/09/04 06:00 [entrez]
PHST- 2014/09/04 06:00 [pubmed]
PHST- 2015/05/15 06:00 [medline]
AID - 10.1002/alr.21384 [doi]
PST - ppublish
SO  - Int Forum Allergy Rhinol. 2014 Sep;4 Suppl 2:S46-50. doi: 10.1002/alr.21384.