PMID- 25183358
OWN - NLM
STAT- MEDLINE
DCOM- 20150714
LR  - 20191210
IS  - 1873-5967 (Electronic)
IS  - 1386-6532 (Linking)
VI  - 61
IP  - 3
DP  - 2014 Nov
TI  - Human cytomegalovirus quantification in toddlers saliva from day care centers and 
      emergency unit: a feasibility study.
PG  - 371-7
LID - S1386-6532(14)00300-X [pii]
LID - 10.1016/j.jcv.2014.07.020 [doi]
AB  - BACKGROUND: Cytomegalovirus (CMV) infection is the most important cause of 
      congenital viral infection in developed countries. In utero transmission occurs 
      at higher rates in seronegative women during primary infection, especially those 
      in contact with young children in day-care centers (DCC). Nevertheless data on 
      variability of CMV excretion among children in French DCCs are lacking, and are 
      important for public health planning. OBJECTIVES: Our main objective was to 
      assess the feasibility of a salivary sample in DCCs in order to study CMV 
      excretion among toddlers. Our secondary aims were to assess prevalence of CMV 
      excretion in children attending Hospital Emergency Unit (EU) in comparison with 
      various types of DCCs and to validate the analytical chain for collected 
      specimens. STUDY DESIGN: Excretion of CMV in saliva was quantified using a 
      real-time PCR assay in children aged from 3 months to 6 years old in EU and in 
      DCC, with gB, gH and gN genotypes determined in infected children. Salivary 
      sampling was performed using small sponges placed into a DNA conservation medium. 
      Socio cultural and medical information were collected from attending parents. 
      RESULTS: A total of 625 children were included, with 256 from six DCCs and 369 
      from one EU. In DCCs, the acceptability of the procedure was 87.3% (95%CI 
      78.5-96.2) amongst parents and children, and in the EU, acceptability was higher 
      at 97.6% (95%CI 95.5-98.9). CMV shedding overall prevalence was 21.7% (95%CI 
      17.6-26.2), with CMV shedding prevalence in DCCs of 51.9% (95%CI 22.8-81.1). 
      CONCLUSION: We validated the feasibility and acceptability of measuring CMV 
      shedding in the saliva of French toddlers. The discrepancy between CMV infection 
      rates in day care centers and in the general population (as sampled in the EU) 
      indicates the need for a further study to determine risk factors and shedding 
      levels in the DCC population.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Grosjean, J
AU  - Grosjean J
AD  - CHU Limoges, Bacteriology-Virology Department, Limoges, France; National 
      Reference Center for Cytomegaloviruses, Limoges, France; Univ Limoges, Inserm UMR 
      1092, Faculte de Medecine, Limoges, France.
FAU - Trapes, L
AU  - Trapes L
AD  - CHU Limoges, Pediatric Emergency Unit, Limoges, France.
FAU - Hantz, S
AU  - Hantz S
AD  - CHU Limoges, Bacteriology-Virology Department, Limoges, France; National 
      Reference Center for Cytomegaloviruses, Limoges, France; Univ Limoges, Inserm UMR 
      1092, Faculte de Medecine, Limoges, France.
FAU - Mengelle, C
AU  - Mengelle C
AD  - CHU Toulouse, Virology Department, Toulouse, France.
FAU - Virey, B
AU  - Virey B
AD  - Pediatrician, Association Francaise de Pediatrie Ambulatoire, Dijon, France.
FAU - Undreiner, F
AU  - Undreiner F
AD  - Pediatrician, Association Francaise de Pediatrie Ambulatoire, Strasbourg, France.
FAU - Messager, V
AU  - Messager V
AD  - CHU Limoges, Pediatric Emergency Unit, Limoges, France.
FAU - Denis, F
AU  - Denis F
AD  - CHU Limoges, Bacteriology-Virology Department, Limoges, France; Univ Limoges, 
      Inserm UMR 1092, Faculte de Medecine, Limoges, France.
FAU - Marin, B
AU  - Marin B
AD  - CHU Limoges, Unite Fonctionnelle de Recherche Clinique et de Biostatistique, 
      Limoges, France.
FAU - Alain, S
AU  - Alain S
AD  - CHU Limoges, Bacteriology-Virology Department, Limoges, France; National 
      Reference Center for Cytomegaloviruses, Limoges, France; Univ Limoges, Inserm UMR 
      1092, Faculte de Medecine, Limoges, France. Electronic address: 
      sophie.alain@unilim.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20140817
PL  - Netherlands
TA  - J Clin Virol
JT  - Journal of clinical virology : the official publication of the Pan American 
      Society for Clinical Virology
JID - 9815671
SB  - IM
MH  - Child
MH  - Child Day Care Centers
MH  - Child, Preschool
MH  - Cytomegalovirus/*isolation & purification
MH  - Cytomegalovirus Infections/*virology
MH  - Emergency Service, Hospital
MH  - Feasibility Studies
MH  - Female
MH  - France
MH  - Humans
MH  - Infant
MH  - Male
MH  - Pilot Projects
MH  - Real-Time Polymerase Chain Reaction
MH  - Saliva/*virology
MH  - *Virus Shedding
OTO - NOTNLM
OT  - Cytomegalovirus, Saliva, Quantification, Day care center
EDAT- 2014/09/04 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/09/04 06:00
PHST- 2014/05/27 00:00 [received]
PHST- 2014/07/25 00:00 [revised]
PHST- 2014/07/30 00:00 [accepted]
PHST- 2014/09/04 06:00 [entrez]
PHST- 2014/09/04 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - S1386-6532(14)00300-X [pii]
AID - 10.1016/j.jcv.2014.07.020 [doi]
PST - ppublish
SO  - J Clin Virol. 2014 Nov;61(3):371-7. doi: 10.1016/j.jcv.2014.07.020. Epub 2014 Aug 
      17.