PMID- 25183945
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140903
LR  - 20211021
IS  - 1319-562X (Print)
IS  - 2213-7106 (Electronic)
IS  - 2213-7106 (Linking)
VI  - 21
IP  - 4
DP  - 2014 Sep
TI  - Biochemical and molecular studies on the possible influence of the Brassica 
      oleracea and Beta vulgaris extracts to mitigate the effect of food preservatives 
      and food chemical colorants on albino rats.
PG  - 342-54
LID - 10.1016/j.sjbs.2013.11.004 [doi]
AB  - This study aimed to investigate the biochemical influence of broccoli and beet 
      extracts on selected individual additives NaNO2 or sunset yellow treated rats, in 
      addition to the gene expression of some antioxidant enzymes. Forty-two male rats 
      were assigned to seven groups of six rats in each group. The control group was 
      fed a diet without an additive for four weeks. Group (2) received NaNO2, groups 
      (3) received NaNO2 co-administered with broccoli extract (4) NaNO2 
      co-administered with beet extracts, Group (5) received sunset yellow, Group (6) 
      received sunset yellow co-administered with broccoli extract, and Group (7) 
      received sunset yellow co-administered with beet extract, for four weeks. At the 
      end of the experiment, blood, liver, kidney, and brain samples were taken for 
      biochemical and/or molecular analysis. The mRNA expression of antioxidant enzymes 
      was determined by reversing transcriptase-polymerase chain reaction (RT-PCR). The 
      obtained results revealed that rats co-administered with beet or broccoli 
      extracts had a significant decrease in serum levels of AST, ALT, ALP, urea, total 
      lipids, and triglycerides, as well as a significant increase in reduced 
      glutathione (GSH), glutathione peroxidase (GSH-px), and superoxide dismutase 
      (SOD) enzyme activities, compared to the normal control group. Oral 
      administration of NaNO2 or sunset yellow caused a significant increase in serum 
      levels of AST, ALT, ALP, urea, total lipids, and triglycerides, as well as a 
      significant decrease in GSH, GSH-px, and SOD compared to the positive group. In 
      conclusion, this study showed that broccoli and beet extracts have a protective 
      effect against NaNO2 or sunset yellow in rat treated groups.
FAU - Sarhan, Mohammed A A
AU  - Sarhan MA
AD  - Molecular Biology Lab., Department of Biology, College of Science, King Khalid 
      University, Saudi Arabia.
FAU - Shati, Ali A
AU  - Shati AA
AD  - Molecular Biology Lab., Department of Biology, College of Science, King Khalid 
      University, Saudi Arabia.
FAU - Elsaid, Fahmy G
AU  - Elsaid FG
AD  - Molecular Biology Lab., Department of Biology, College of Science, King Khalid 
      University, Saudi Arabia ; Zoology Department, Faculty of Science, Mansoura 
      University, Mansoura, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20131128
PL  - Saudi Arabia
TA  - Saudi J Biol Sci
JT  - Saudi journal of biological sciences
JID - 101543796
PMC - PMC4150234
OTO - NOTNLM
OT  - Antioxidant enzymes
OT  - Beet
OT  - Broccoli
OT  - Gene expression
OT  - Sodium nitrite
OT  - Sunset yellow
EDAT- 2014/09/04 06:00
MHDA- 2014/09/04 06:01
PMCR- 2013/11/28
CRDT- 2014/09/04 06:00
PHST- 2013/07/25 00:00 [received]
PHST- 2013/11/10 00:00 [revised]
PHST- 2013/11/13 00:00 [accepted]
PHST- 2014/09/04 06:00 [entrez]
PHST- 2014/09/04 06:00 [pubmed]
PHST- 2014/09/04 06:01 [medline]
PHST- 2013/11/28 00:00 [pmc-release]
AID - S1319-562X(13)00101-0 [pii]
AID - 10.1016/j.sjbs.2013.11.004 [doi]
PST - ppublish
SO  - Saudi J Biol Sci. 2014 Sep;21(4):342-54. doi: 10.1016/j.sjbs.2013.11.004. Epub 
      2013 Nov 28.