PMID- 25184138
OWN - NLM
STAT- MEDLINE
DCOM- 20150511
LR  - 20220409
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2014
DP  - 2014
TI  - miR-375 suppresses IGF1R expression and contributes to inhibition of cell 
      progression in laryngeal squamous cell carcinoma.
PG  - 374598
LID - 10.1155/2014/374598 [doi]
LID - 374598
AB  - MicroRNAs (miRNAs) are small noncoding RNA molecules which are involved in 
      tumorigenesis and development. To investigate their role in primary laryngeal 
      squamous cell carcinoma (LSCC), miRNA GeneChips were used to screen the 
      differentially expressed miRNA, and then validated by real-time quantitative PCR 
      in LSCC samples, we found that miR-375 was frequently downregulated in primary 
      LSCC tissues. The tumor-suppressive effect of miR-375 was determined by in vitro 
      assays; through gain-of-function studies we demonstrated that miR-375 can inhibit 
      LSCC cell (SNU-48 and SNU-899) proliferation, motility, and invasion, and promote 
      their apoptosis. In addition, bioinformatics tools TargetScan, PicTar, and 
      Miranda were used to investigate the potential target of miR-375; bioinformatics 
      analysis and dual-luciferase reporter assay indicated that IGF1R was a novel 
      direct target of miR-375. Ectopic transfection of miR-375 led to a significant 
      reduction in IGF1R and its downstream signaling molecule AKT at both the mRNA and 
      protein levels in LSCC cells. Our results suggested that downregulation of 
      miR-375 is one of the molecular mechanisms for the development and progression of 
      LSCC by directly targeting IGF1R and affecting its downstream AKT signaling 
      pathways. Furthermore, miR-375 and IGF1R may serve as a novel therapeutic target 
      for LSCC.
FAU - Luo, Jie
AU  - Luo J
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen 
      University, Otorhinolaryngology Institute, Sun Yat-sen University, Guangzhou, 
      China.
FAU - Wu, Jianhui
AU  - Wu J
AUID- ORCID: 0000-0003-4016-183X
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen 
      University, Otorhinolaryngology Institute, Sun Yat-sen University, Guangzhou, 
      China.
FAU - Li, Zenghong
AU  - Li Z
AD  - Department of Surgery, Queen Mary Hospital, The University of Hong Kong, Hong 
      Kong.
FAU - Qin, Hao
AU  - Qin H
AD  - Department of Otorhinolaryngology, The First People's Hospital of Foshan, Foshan, 
      China.
FAU - Wang, Bin
AU  - Wang B
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen 
      University, Otorhinolaryngology Institute, Sun Yat-sen University, Guangzhou, 
      China.
FAU - Wong, Thian-Sze
AU  - Wong TS
AD  - Department of Surgery, Queen Mary Hospital, The University of Hong Kong, Hong 
      Kong.
FAU - Yang, Weiqiang
AU  - Yang W
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen 
      University, Otorhinolaryngology Institute, Sun Yat-sen University, Guangzhou, 
      China.
FAU - Fu, Qing-Ling
AU  - Fu QL
AD  - Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen 
      University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, China.
FAU - Lei, Wenbin
AU  - Lei W
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen 
      University, Otorhinolaryngology Institute, Sun Yat-sen University, Guangzhou, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140812
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (IGF1 protein, human)
RN  - 0 (IGF1R protein, human)
RN  - 0 (MIRN375 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Receptors, Somatomedin)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 2.7.10.1 (Receptor, IGF Type 1)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
SB  - IM
MH  - Apoptosis
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Cell Transformation, Neoplastic/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Insulin-Like Growth Factor I/*biosynthesis/genetics
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - MicroRNAs/*genetics/metabolism
MH  - Oncogene Protein v-akt/biosynthesis
MH  - Receptor, IGF Type 1
MH  - Receptors, Somatomedin/*biosynthesis
MH  - Signal Transduction
PMC - PMC4145380
EDAT- 2014/09/04 06:00
MHDA- 2015/05/12 06:00
PMCR- 2014/08/12
CRDT- 2014/09/04 06:00
PHST- 2014/03/16 00:00 [received]
PHST- 2014/06/17 00:00 [revised]
PHST- 2014/06/24 00:00 [accepted]
PHST- 2014/09/04 06:00 [entrez]
PHST- 2014/09/04 06:00 [pubmed]
PHST- 2015/05/12 06:00 [medline]
PHST- 2014/08/12 00:00 [pmc-release]
AID - 10.1155/2014/374598 [doi]
PST - ppublish
SO  - Biomed Res Int. 2014;2014:374598. doi: 10.1155/2014/374598. Epub 2014 Aug 12.