PMID- 25184992
OWN - NLM
STAT- MEDLINE
DCOM- 20150226
LR  - 20141103
IS  - 1522-1555 (Electronic)
IS  - 0193-1849 (Linking)
VI  - 307
IP  - 9
DP  - 2014 Nov 1
TI  - Alterations in the profile of blood neutrophil membrane receptors caused by in 
      vivo adrenocorticotrophic hormone actions.
PG  - E754-63
LID - 10.1152/ajpendo.00227.2014 [doi]
AB  - Elevated levels of adrenocorticotrophic hormone (ACTH) mobilize granulocytes from 
      bone marrow into the blood, although these neutrophils are refractory to a full 
      migratory response into inflamed tissues. Here, we investigated the dependence of 
      glucocorticoid receptor activation and glucocorticoid-regulated protein annexin 
      A1 (ANXA1) on ACTH-induced neutrophilia and the phenotype of blood neutrophil 
      after ACTH injection, focusing on adhesion molecule expressions and locomotion 
      properties. ACTH injection (5 mug ip, 4 h) induced neutrophilia in wild-type (WT) 
      mice and did not alter the elevated numbers of neutrophils in RU-38486 
      (RU)-pretreated or ANXA1(-/-) mice injected with ACTH. Neutrophils from WT 
      ACTH-treated mice presented higher expression of Ly6G(+)ANXA1(high), CD18(high), 
      CD62L(high), CD49(high), CXCR4(high), and formyl-peptide receptor 1 (FPR1(low)) 
      than those observed in RU-pretreated or ANXA1(-/-) mice. The membrane phenotype 
      of neutrophils collected from WT ACTH-treated mice was paralleled by elevated 
      fractions of rolling and adherent leukocytes to the cremaster postcapillary 
      venules together with impaired neutrophil migration into inflamed air pouches in 
      vivo and in vitro reduced formyl-methionyl-leucyl-phenylalanine (fMLP) or 
      stromal-derived factor-1 (SDF-1alpha)-induced chemotaxis. In an 18-h senescence 
      protocol, neutrophils from WT ACTH-treated mice had a higher proportion of 
      ANXAV(low)/CXCR4(low), and they were less phagocytosed by peritoneal macrophages. 
      We conclude that alterations on HPA axis affect the pattern of membrane receptors 
      in circulating neutrophils, which may lead to different neutrophil phenotypes in 
      the blood. Moreover, ACTH actions render circulating neutrophils to a phenotype 
      with early reactivity, such as in vivo leukocyte-endothelial interactions, but 
      with impaired locomotion and clearance.
CI  - Copyright (c) 2014 the American Physiological Society.
FAU - Machado, Isabel Daufenback
AU  - Machado ID
AD  - Department of Clinical and Toxicological Analyses, Faculty of Pharmaceutical 
      Sciences, University of Sao Paulo, Sao Paulo, Brazil;
FAU - Santin, Jose Roberto
AU  - Santin JR
AD  - Department of Clinical and Toxicological Analyses, Faculty of Pharmaceutical 
      Sciences, University of Sao Paulo, Sao Paulo, Brazil;
FAU - Drewes, Carine Cristiane
AU  - Drewes CC
AD  - Department of Clinical and Toxicological Analyses, Faculty of Pharmaceutical 
      Sciences, University of Sao Paulo, Sao Paulo, Brazil;
FAU - Gil, Cristiane Damas
AU  - Gil CD
AD  - Department of Morphology and Genetics, Federal University of Sao Paulo, Sao 
      Paulo, Brazil;
FAU - Oliani, Sonia Maria
AU  - Oliani SM
AD  - Department of Biology, Instituto de Biociencias, Letras e Ciencias Exatas, Sao 
      Paulo State University, Sao Jose do Rio Preto, Brazil; and.
FAU - Perretti, Mauro
AU  - Perretti M
AD  - The William Harvey Research Institute, Barts and The London School of Medicine, 
      Queen Mary University of London, London, United Kingdom.
FAU - Farsky, Sandra Helena Poliselli
AU  - Farsky SH
AD  - Department of Clinical and Toxicological Analyses, Faculty of Pharmaceutical 
      Sciences, University of Sao Paulo, Sao Paulo, Brazil; sfarsky@usp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140902
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Annexin A1)
RN  - 0 (Hormone Antagonists)
RN  - 0 (Receptors, Corticotropin)
RN  - 0 (annexin A1, mouse)
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Adrenocorticotropic Hormone/administration & dosage/antagonists & 
      inhibitors/blood/*metabolism
MH  - Animals
MH  - Annexin A1/blood/genetics/*metabolism
MH  - Cell Membrane/drug effects/metabolism
MH  - Cells, Cultured
MH  - Chemotaxis, Leukocyte/drug effects
MH  - Corticosterone/blood/metabolism
MH  - Hormone Antagonists/pharmacology
MH  - *Leukopoiesis/drug effects
MH  - Macrophages/drug effects/immunology/metabolism/pathology
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Mice, Knockout
MH  - Neutrophils/drug effects/immunology/*metabolism/pathology
MH  - Phagocytosis/drug effects
MH  - Receptors, Corticotropin/agonists/antagonists & inhibitors/blood/*metabolism
MH  - *Stress, Physiological/drug effects
MH  - Stress, Psychological/blood/*immunology/metabolism/pathology
MH  - Surface Properties/drug effects
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - adhesion molecules
OT  - chemokine (C-X-C motif) receptor 4
OT  - chemotaxis
OT  - hypothalamic-pituitary-adrenal axis
OT  - intravital microscopy
EDAT- 2014/09/04 06:00
MHDA- 2015/02/27 06:00
CRDT- 2014/09/04 06:00
PHST- 2014/09/04 06:00 [entrez]
PHST- 2014/09/04 06:00 [pubmed]
PHST- 2015/02/27 06:00 [medline]
AID - ajpendo.00227.2014 [pii]
AID - 10.1152/ajpendo.00227.2014 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2014 Nov 1;307(9):E754-63. doi: 
      10.1152/ajpendo.00227.2014. Epub 2014 Sep 2.