PMID- 25185066
OWN - NLM
STAT- MEDLINE
DCOM- 20150605
LR  - 20211021
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 19
IP  - 9
DP  - 2014 Sep 2
TI  - Peptides derived from Rhopilema esculentum hydrolysate exhibit angiotensin 
      converting enzyme (ACE) inhibitory and antioxidant abilities.
PG  - 13587-602
LID - 10.3390/molecules190913587 [doi]
AB  - Jellyfish (Rhopilema esculentum) was hydrolyzed using alcalase, and two peptides 
      with angiotensin-I-converting enzyme (ACE) inhibitory and antioxidant activities 
      were purified by ultrafiltration and consecutive chromatographic methods. The 
      amino acid sequences of the two peptides were identified as VKP (342 Da) and 
      VKCFR (651 Da) by electrospray ionization tandem mass spectrometry. The IC50 
      values of ACE inhibitory activities of the two peptides were 1.3 muM and 34.5 muM, 
      respectively. Molecular docking results suggested that VKP and VKCFR bind to ACE 
      through coordinating with the active site Zn(II) atom. Free radical scavenging 
      activity and protection against hydrogen peroxide (H2O2)-induced rat cerebral 
      microvascular endothelial cell (RCMEC) injury were used to evaluate the 
      antioxidant activities of the two peptides. As the results clearly showed that 
      the peptides increased the superoxide dismutase (SOD), catalase (CAT) and 
      glutathione peroxidase (GSH-px) activities in RCMEC cells), it is proposed that 
      the R. esculentum peptides exert significant antioxidant effects.
FAU - Li, Jun
AU  - Li J
AD  - State Key Laboratory of Reproductive Medicine, Department of Plastic & Consmetic 
      Surgery, Nanjing Maternity and Child Health Hospital, Nanjing Medical University, 
      Nanjing 210029, China. drlijun@126.com.
FAU - Li, Qian
AU  - Li Q
AD  - State Key Laboratory of Reproductive Medicine, Department of Plastic & Consmetic 
      Surgery, Nanjing Maternity and Child Health Hospital, Nanjing Medical University, 
      Nanjing 210029, China. drliqian@126.com.
FAU - Li, Jingyun
AU  - Li J
AD  - State Key Laboratory of Reproductive Medicine, Department of Plastic & Consmetic 
      Surgery, Nanjing Maternity and Child Health Hospital, Nanjing Medical University, 
      Nanjing 210029, China. lijingyun175@gmail.com.
FAU - Zhou, Bei
AU  - Zhou B
AD  - State Key Laboratory of Reproductive Medicine, Department of Plastic & Consmetic 
      Surgery, Nanjing Maternity and Child Health Hospital, Nanjing Medical University, 
      Nanjing 210029, China. enzhoubei@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140902
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Angiotensin-Converting Enzyme Inhibitors)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Peptide Fragments)
RN  - EC 3.4.15.1 (Peptidyl-Dipeptidase A)
SB  - IM
MH  - Amino Acid Sequence
MH  - Angiotensin-Converting Enzyme Inhibitors/*chemistry/isolation & 
      purification/pharmacology
MH  - Animals
MH  - Cells, Cultured
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Reverse-Phase
MH  - Endothelial Cells/drug effects/metabolism
MH  - Free Radical Scavengers/*chemistry/isolation & purification/pharmacology
MH  - Hydrolysis
MH  - Molecular Docking Simulation
MH  - Peptide Fragments/*chemistry/isolation & purification/pharmacology
MH  - Peptidyl-Dipeptidase A/chemistry
MH  - Rats
MH  - Scyphozoa/chemistry
PMC - PMC6271940
COIS- The authors declare no conflict of interest.
EDAT- 2014/09/04 06:00
MHDA- 2015/06/06 06:00
PMCR- 2014/09/02
CRDT- 2014/09/04 06:00
PHST- 2014/07/08 00:00 [received]
PHST- 2014/08/22 00:00 [revised]
PHST- 2014/08/26 00:00 [accepted]
PHST- 2014/09/04 06:00 [entrez]
PHST- 2014/09/04 06:00 [pubmed]
PHST- 2015/06/06 06:00 [medline]
PHST- 2014/09/02 00:00 [pmc-release]
AID - molecules190913587 [pii]
AID - molecules-19-13587 [pii]
AID - 10.3390/molecules190913587 [doi]
PST - epublish
SO  - Molecules. 2014 Sep 2;19(9):13587-602. doi: 10.3390/molecules190913587.