PMID- 25185312
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 32
IP  - 5
DP  - 2014 May
TI  - [Determination of biurea in flour and its products by liquid 
      chromatography-tandem mass spectrometry].
PG  - 513-8
AB  - A novel method was established for the determination and identification of biurea 
      in flour and its products using liquid chromatography-tandem mass spectrometry 
      (LC-MS/MS). The biurea was extracted with water and oxidized to azodicarbonamide 
      by potassium permanganate. The azodicarbonamide was then derivatized using sodium 
      p-toluene sulfinate solution. The separation was performed on a Shimpak XR-ODS II 
      column (150 mm x 2.0 mm, 2.2 microm) using the mobile phase composed of 
      acetonitrile and 2 mmol/L ammonium acetate aqueous solution (containing 0.2% 
      (v/v) formic acid) with a gradient elution program. Tandem mass spectrometric 
      detection was performed in multiple reaction monitoring (MRM) scan mode with a 
      positive electrospray ionization (ESI(+)) source. The method used stable isotope 
      internal standard quantitation. The calibration curve showed good linearity over 
      the range of 1-20 000 microg/kg (R2 = 0.999 9). The limit of quantification was 5 
      microg/kg for biurea spiked in flour and its products. At the spiking levels of 
      5.0, 10.0 and 50.0 microg/kg in different matrices, the average recovery o biurea 
      was 78.3%-108.0% with the relative standard deviations (RSDs) < or = 5.73%. The 
      method developed is novel, reliable and sensitive with wide linear range, and can 
      be used to determine the biurea in flour and its products.
FAU - Wang, Ya
AU  - Wang Y
FAU - Wang, Junsu
AU  - Wang J
FAU - Xiang, Lu
AU  - Xiang L
FAU - Xi, Cunxian
AU  - Xi C
FAU - Chen, Dongdong
AU  - Chen D
FAU - Peng, Tao
AU  - Peng T
FAU - Wang, Guomin
AU  - Wang G
FAU - Mu, Zhaode
AU  - Mu Z
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Biureas)
SB  - IM
MH  - Biureas/*analysis
MH  - Chromatography, Liquid
MH  - Flour/*analysis
MH  - Food Contamination/*analysis
MH  - Tandem Mass Spectrometry
EDAT- 2014/09/05 06:00
MHDA- 2015/09/02 06:00
CRDT- 2014/09/05 06:00
PHST- 2014/09/05 06:00 [entrez]
PHST- 2014/09/05 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.3724/sp.j.1123.2013.12023 [doi]
PST - ppublish
SO  - Se Pu. 2014 May;32(5):513-8. doi: 10.3724/sp.j.1123.2013.12023.