PMID- 25185313
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 32
IP  - 5
DP  - 2014 May
TI  - [Determination of cefalonium residue in milk by high performance liquid 
      chromatography-tandem mass spectrometry].
PG  - 519-23
AB  - An analytical method was developed for the determination of cefalonium in milk by 
      high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). A 
      total of 1 g milk was deproteinized by acetonitrile. The supernatant was 
      transferred into a test tube to be blown dry with N2 at 37 degrees C. Then the 
      residue was dissolved with methanol-0.1% formic acid in water (3:7, v/v). The 
      sample was determined by HPLC-MS/MS after the purification. The chromatographic 
      separation was achieved on a C18 column using 0.1% formic acid in water and 
      acetonitrile as mobile phases with gradient elution. Qualitative and quantitative 
      analyses were achieved by HPLC-MS/MS under positive ionization and multiple 
      reaction monitoring (MRM) mode. Matrix-matched calibration curve was used for the 
      quantification. Good correlation coefficients were obtained (r > 0. 999) in the 
      mass concentration range of 2-200 microg/L. The limit of detection (LOD, S/N > or 
      = 3) was 0.5 microg/kg in milk, and the limit of quantification (LOQ, S/N 10) was 
      2 microg/kg. The mean recoveries at the four levels of LOQ, 1/2MRL (maximum 
      residue level), MRL, 2MRL were between 78.5% and 86.2%, with the intra-day 
      relative standard deviations (RSDs) of 1.5% to 6.2% and inter-day RSDs of 2.9% to 
      5.6%. In conclusion, the established method can be applied for the determination 
      of cefalonium residue in milk.
FAU - Li, Shuaipeng
AU  - Li S
FAU - Guo, Chunna
AU  - Guo C
FAU - Meng, Lei
AU  - Meng L
FAU - Huang, Xianhui
AU  - Huang X
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Cephalosporins)
SB  - IM
MH  - Animals
MH  - Cephalosporins/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Drug Residues/*analysis
MH  - Food Contamination/*analysis
MH  - Milk/*chemistry
MH  - Tandem Mass Spectrometry
EDAT- 2014/09/05 06:00
MHDA- 2015/09/02 06:00
CRDT- 2014/09/05 06:00
PHST- 2014/09/05 06:00 [entrez]
PHST- 2014/09/05 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.3724/sp.j.1123.2013.12028 [doi]
PST - ppublish
SO  - Se Pu. 2014 May;32(5):519-23. doi: 10.3724/sp.j.1123.2013.12028.