PMID- 25186037
OWN - NLM
STAT- MEDLINE
DCOM- 20150609
LR  - 20191210
IS  - 1944-0057 (Electronic)
IS  - 1944-0057 (Linking)
VI  - 31
IP  - 10
DP  - 2014
TI  - Evaluation and validation of biochip multi-array technology for the screening of 
      six families of antibiotics in honey according to the European guideline for the 
      validation of screening methods for residues of veterinary medicines.
PG  - 1699-711
LID - 10.1080/19440049.2014.952784 [doi]
AB  - The main chemicals used against varoa are acaricides, and the antibiotics used 
      for the control of bee bacterial diseases are mainly tetracyclines, 
      streptomycins, sulfonamides and chloramphenicol. No maximum residue limits (MRLs) 
      have been set for any antibiotics in honey. Therefore, in the European Union, 
      minimum recommended concentrations (RC) for the analytical performance of methods 
      to control a certain set of these non-authorised chemicals in honey were 
      published by the European Union Reference Laboratory (EU-RL) in 2007. Concerning 
      the strategy for the control for antibiotic residues in honey, there is still a 
      great need for a cheap and single multi-residue method. Biochip array technology 
      is an innovative assay technology for the multi-analyte screening of biological 
      samples in a rapid and easy-to-use format. A multi-array system, called Evidence 
      Investigator (Randox, Crumlin, Co., Antrim, UK), was evaluated in our 
      laboratory. It is a semi-automated biochip system designed for research, clinical 
      applications and veterinary use. A competitive chemiluminescent immunoassay is 
      employed for the detection of antimicrobials. The MicroArray II kit (AM II) 
      dedicated to the screening of six different families of antibiotic residues was 
      validated according to the European guideline for the validation of screening 
      methods for residues of veterinary medicines. The specificity was proven to be 
      very satisfactory, and applicability to different kinds of honey was 
      demonstrated. The detection capabilities (CCbeta) of six antibiotic residues were 
      determined and were below the RCs when exist. The AM II kit could detect at least 
      six quinolones, four tetracyclines and three epimers, three aminoglycosides, 
      three macrolides, thiamphenicol, florfenicol and ceftiofur along with one of its 
      stabilised metabolites, the desfuroylceftiofurcysteine disulfide (DCCD).
FAU - Gaudin, Valerie
AU  - Gaudin V
AD  - a ANSES, Laboratoire de Fougeres , Fougeres Cedex , France.
FAU - Hedou, Celine
AU  - Hedou C
FAU - Soumet, Christophe
AU  - Soumet C
FAU - Verdon, Eric
AU  - Verdon E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess
JT  - Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & 
      risk assessment
JID - 101485040
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antibodies, Immobilized)
RN  - 0 (Veterinary Drugs)
SB  - IM
MH  - Anti-Bacterial Agents/*analysis
MH  - Antibodies, Immobilized/metabolism
MH  - Antibody Specificity
MH  - Automation, Laboratory
MH  - Cross Reactions
MH  - Drug Residues/*analysis
MH  - European Union
MH  - False Positive Reactions
MH  - *Food Contamination
MH  - Food Inspection/*methods/standards
MH  - Guideline Adherence
MH  - Guidelines as Topic
MH  - Honey/*analysis
MH  - Limit of Detection
MH  - Protein Array Analysis
MH  - Quality Control
MH  - Reproducibility of Results
MH  - Veterinary Drugs/*analysis
OTO - NOTNLM
OT  - Decision No. 2002/657/EC
OT  - antibiotic, honey
OT  - biochip
OT  - multi-array
OT  - screening
OT  - validation
EDAT- 2014/09/05 06:00
MHDA- 2015/06/10 06:00
CRDT- 2014/09/05 06:00
PHST- 2014/09/05 06:00 [entrez]
PHST- 2014/09/05 06:00 [pubmed]
PHST- 2015/06/10 06:00 [medline]
AID - 10.1080/19440049.2014.952784 [doi]
PST - ppublish
SO  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 
      2014;31(10):1699-711. doi: 10.1080/19440049.2014.952784.