PMID- 25186098
OWN - NLM
STAT- MEDLINE
DCOM- 20151124
LR  - 20191210
IS  - 1099-1263 (Electronic)
IS  - 0260-437X (Linking)
VI  - 35
IP  - 4
DP  - 2015 Apr
TI  - Immunophenotyping does not improve predictivity of the local lymph node assay in 
      mice.
PG  - 434-45
LID - 10.1002/jat.3042 [doi]
AB  - The local lymph node assay (LLNA) is a regulatory accepted test for the 
      identification of skin sensitizing substances by measuring radioactive thymidine 
      incorporation into the lymph node. However, there is evidence that LLNA is 
      overestimating the sensitization potential of certain substance classes in 
      particular those exerting skin irritation. Some reports describe the additional 
      use of flow cytometry-based immunophenotyping to better discriminate irritants 
      from sensitizing irritants in LLNA. In the present study, the 22 performance 
      standards plus 8 surfactants were assessed using the radioactive LLNA method. In 
      addition, lymph node cells were immunophenotyped to evaluate the specificity of 
      the lymph node response using cell surface markers such as B220 or CD19, CD3, 
      CD4, CD8, I-A(kappa) and CD69 with the aim to allow a better discrimination above all 
      between irritants and sensitizers, but also non-irritating sensitizers and 
      non-sensitizers. However, the markers assessed in this study do not sufficiently 
      differentiate between irritants and irritant sensitizers and therefore did not 
      improve the predictive capacity of the LLNA.
CI  - Copyright (c) 2014 John Wiley & Sons, Ltd.
FAU - Strauss, Volker
AU  - Strauss V
AD  - BASF SE, Experimental Toxicology and Ecology, Z470, 67056, Ludwigshafen, Germany.
FAU - Kolle, Susanne N
AU  - Kolle SN
FAU - Honarvar, Naveed
AU  - Honarvar N
FAU - Dammann, Martina
AU  - Dammann M
FAU - Groeters, Sibylle
AU  - Groeters S
FAU - Faulhammer, Frank
AU  - Faulhammer F
FAU - Landsiedel, Robert
AU  - Landsiedel R
FAU - van Ravenzwaay, Bennard
AU  - van Ravenzwaay B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Validation Study
DEP - 20140903
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Antigens, Surface)
RN  - 0 (Haptens)
RN  - 0 (Irritants)
RN  - 0 (Surface-Active Agents)
SB  - IM
MH  - Animals
MH  - Antigens, Surface/metabolism
MH  - Dermatitis, Contact/immunology/metabolism
MH  - Female
MH  - Haptens/*toxicity
MH  - Immunophenotyping
MH  - Irritants/*toxicity
MH  - Local Lymph Node Assay
MH  - Lymph Nodes/*drug effects/immunology/metabolism
MH  - Lymphocytes/*drug effects/immunology/metabolism
MH  - Mice, Inbred CBA
MH  - *Models, Biological
MH  - Surface-Active Agents/chemistry
OTO - NOTNLM
OT  - LLNA
OT  - immunophenotyping
OT  - mice
OT  - predicitivity
OT  - skin sensitization
EDAT- 2014/09/05 06:00
MHDA- 2015/12/15 06:00
CRDT- 2014/09/05 06:00
PHST- 2014/03/24 00:00 [received]
PHST- 2014/06/05 00:00 [revised]
PHST- 2014/06/05 00:00 [accepted]
PHST- 2014/09/05 06:00 [entrez]
PHST- 2014/09/05 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1002/jat.3042 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2015 Apr;35(4):434-45. doi: 10.1002/jat.3042. Epub 2014 Sep 3.