PMID- 25186625
OWN - NLM
STAT- MEDLINE
DCOM- 20150423
LR  - 20211021
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 45
IP  - 1
DP  - 2014 Sep 4
TI  - The comparative profile of lymphoid cells and the T and B cell spectratype of 
      germ-free piglets infected with viruses SIV, PRRSV or PCV2.
PG  - 91
LID - 10.1186/s13567-014-0091-x [doi]
LID - 91
AB  - Lymphocyte subsets isolated from germ-free piglets experimentally infected with 
      swine influenza virus (SIV), porcine reproductive and respiratory syndrome virus 
      (PRRSV) or porcine circovirus type 2 (PCV2) were studied and the profile of these 
      subsets among these three infections was monitored. Germ-free piglets were used 
      since their response could be directly correlated to the viral infection. Because 
      SIV infections are resolved even by colostrum-deprived neonates whereas PRRSV and 
      PCV2 infections are not, SIV was used as a benchmark for an effectively resolved 
      viral infection. PRRSV caused a large increase in the proportion of lymphocytes 
      at the site of infection and rapid differentiation of B cells leading to a high 
      level of Ig-producing cells but a severe reduction in CD2-CD21+ primed B cells. 
      Unlike SIV and PCV2, PRRSV also caused an increase in terminally differentiated 
      subset of CD2+CD8alpha+ gammadelta cells and polyclonal expansion of major Vbeta families 
      suggesting that non-specific helper T cells drive swift B cell activation. 
      Distinct from infections with SIV and PRRSV, PCV2 infection led to the: (a) 
      prevalence of MHC-II+ T cytotoxic cells, (b) restriction of the T helper 
      compartment in the respiratory tract, (c) generation of a high proportion of 
      FoxP3+ T cells in the blood and (d) selective expansion of IgA and IgE suggesting 
      this virus elicits a mucosal immune response. Our findings suggest that PRRSV and 
      PCV2 may negatively modulate the host immune system by different mechanisms which 
      may explain their persistence.
FAU - Sinkora, Marek
AU  - Sinkora M
FAU - Butler, John E
AU  - Butler JE
FAU - Lager, Kelly M
AU  - Lager KM
FAU - Potockova, Hana
AU  - Potockova H
FAU - Sinkorova, Jana
AU  - Sinkorova J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140904
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/*virology
MH  - Circoviridae Infections/*immunology/virology
MH  - Circovirus/physiology
MH  - *Germ-Free Life
MH  - Killer Cells, Natural/*virology
MH  - Orthomyxoviridae/physiology
MH  - Orthomyxoviridae Infections/*immunology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/physiology
MH  - Swine
MH  - T-Lymphocytes/*virology
PMC - PMC4156959
EDAT- 2014/09/05 06:00
MHDA- 2015/04/24 06:00
PMCR- 2014/09/04
CRDT- 2014/09/05 06:00
PHST- 2014/03/17 00:00 [received]
PHST- 2014/08/18 00:00 [accepted]
PHST- 2014/09/05 06:00 [entrez]
PHST- 2014/09/05 06:00 [pubmed]
PHST- 2015/04/24 06:00 [medline]
PHST- 2014/09/04 00:00 [pmc-release]
AID - s13567-014-0091-x [pii]
AID - 91 [pii]
AID - 10.1186/s13567-014-0091-x [doi]
PST - epublish
SO  - Vet Res. 2014 Sep 4;45(1):91. doi: 10.1186/s13567-014-0091-x.