PMID- 25187153
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140905
LR  - 20211021
IS  - 0959-3993 (Print)
IS  - 0959-3993 (Linking)
VI  - 27
IP  - 6
DP  - 2011 Jun
TI  - Molecular differentiation of nocturnally periodic and diurnally sub-periodic 
      Wuchereria bancrofti by Randomly Amplified Polymorphic DNA (RAPD).
PG  - 1525-30
LID - 10.1007/s11274-010-0601-6 [doi]
AB  - Wuchereria bancrofti, a nematode parasite, is responsible for causing 90% of 
      lymphatic filariasis infection in the world. In India, W. bancrofti exists in two 
      physiological forms, nocturnally periodic in the main land and diurnally 
      sub-periodic in Car Nicobar group of islands. Differentiation of these two 
      parasitic forms by conventional microscopic methods difficult requiring good 
      skill and hence tedious. Therefore, we developed a simple and rapid Random 
      Amplified Polymorphic DNA (RAPD) assay to differentiate these parasitic forms. 
      Also, the phylogenetic relationship between periodic and sub-periodic W. 
      bancrofti and also Brugia malayi populations was analyzed using RAPD profile 
      generated. Distinct RAPD profiles were observed among the three parasites with 
      the formation of three distinct clusters in the phylogenetic tree. Substantial 
      genetic diversity (Nei's genetic diversity H) was observed among periodic 
      (H = 0.0577) and sub-periodic (H = 0.1415) W. bancrofti populations.
FAU - Das, M K
AU  - Das MK
AD  - National Institute of Malaria Research (ICMR), Ranchi, India.
FAU - Dhamodharan, R
AU  - Dhamodharan R
FAU - Hoti, S L
AU  - Hoti SL
FAU - Dash, A P
AU  - Dash AP
LA  - eng
PT  - Journal Article
DEP - 20101109
PL  - Germany
TA  - World J Microbiol Biotechnol
JT  - World journal of microbiology & biotechnology
JID - 9012472
EDAT- 2011/06/01 00:00
MHDA- 2011/06/01 00:01
CRDT- 2014/09/05 06:00
PHST- 2010/06/24 00:00 [received]
PHST- 2010/10/22 00:00 [accepted]
PHST- 2014/09/05 06:00 [entrez]
PHST- 2011/06/01 00:00 [pubmed]
PHST- 2011/06/01 00:01 [medline]
AID - 10.1007/s11274-010-0601-6 [doi]
PST - ppublish
SO  - World J Microbiol Biotechnol. 2011 Jun;27(6):1525-30. doi: 
      10.1007/s11274-010-0601-6. Epub 2010 Nov 9.