PMID- 25187602
OWN - NLM
STAT- MEDLINE
DCOM- 20150102
LR  - 20200304
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Linking)
VI  - 63
IP  - Pt 11
DP  - 2014 Nov
TI  - Genetic relatedness among vaginal and anal isolates of Candida albicans from 
      women with vulvovaginal candidiasis in north-east Brazil.
PG  - 1436-1445
LID - 10.1099/jmm.0.076604-0 [doi]
AB  - Vulvovaginal candidiasis (VVC) is one of the most common causes of vaginitis and 
      affects about 75% of women of reproductive age. In order to better understand the 
      epidemiology and pathogenesis of this disease, we evaluated genetic relatedness 
      among 62 clinical isolates of Candida albicans sequentially obtained from the 
      anus and vagina of patients with sporadic and recurrent VVC. Evaluation of 
      patients' demographic and clinical data, direct examination, and colony forming 
      units (c.f.u.) counts of vaginal and anal samples were also performed. The 
      genotypes of strains were determined with ABC genotyping and Randomly Amplified 
      Polymorphic DNA (RAPD). Genotype A was the most prevalent (93.6%), followed by 
      genotype C (6.4%), whereas genotype B was not found. We found the maintenance of 
      the same ABC genotype, regardless of the body site of each patient. Most of the 
      vaginal strains suffered microevolution, whereas most of the anal strains were 
      replaced during the period of study. Vaginal and anal isolates of C. albicans 
      obtained simultaneously from the same patient showed the same ABC genotype and 
      high genetic similarity as determined by RAPD. Genotype A seemed to be dominant 
      in both vaginal and anal isolates of patients with VVC. Our results corroborate 
      the hypothesis that there are 'substrains' of the C. albicans vaginal clone 
      successfully established, which dominate in an apparently random manner over the 
      course of time. It is suggested that the anal reservoir constitutes a possible 
      source for vaginal infection in most of the cases.
CI  - (c) 2014 The Authors.
FAU - Araujo Paulo de Medeiros, Mariana
AU  - Araujo Paulo de Medeiros M
AD  - Department of Clinical and Toxicological Analysis, Federal University of Rio 
      Grande do Norte, Natal, Rio Grande do Norte, Brazil.
FAU - Vieira de Melo, Ana Patricia
AU  - Vieira de Melo AP
AD  - Department of Clinical and Toxicological Analysis, Federal University of Rio 
      Grande do Norte, Natal, Rio Grande do Norte, Brazil.
FAU - Goncalves, Sarah Santos
AU  - Goncalves SS
AD  - Department of Medicine, Federal University of Sao Paulo, Sao Paulo, Brazil.
FAU - Milan, Eveline Pipolo
AU  - Milan EP
AD  - Department of Infectology, Federal University of Rio Grande do Norte, Natal, Rio 
      Grande do Norte, Brazil.
FAU - Chaves, Guilherme Maranhao
AU  - Chaves GM
AD  - Department of Clinical and Toxicological Analysis, Federal University of Rio 
      Grande do Norte, Natal, Rio Grande do Norte, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140903
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
SB  - IM
MH  - Adult
MH  - Anal Canal/*microbiology
MH  - Brazil/epidemiology
MH  - Candida albicans/*genetics/isolation & purification
MH  - Candidiasis, Vulvovaginal/epidemiology/*microbiology
MH  - Female
MH  - Genetic Variation
MH  - Genotype
MH  - Humans
MH  - Prevalence
MH  - Vagina/*microbiology
EDAT- 2014/09/05 06:00
MHDA- 2015/01/03 06:00
CRDT- 2014/09/05 06:00
PHST- 2014/09/05 06:00 [entrez]
PHST- 2014/09/05 06:00 [pubmed]
PHST- 2015/01/03 06:00 [medline]
AID - 10.1099/jmm.0.076604-0 [doi]
PST - ppublish
SO  - J Med Microbiol. 2014 Nov;63(Pt 11):1436-1445. doi: 10.1099/jmm.0.076604-0. Epub 
      2014 Sep 3.