PMID- 25190186
OWN - NLM
STAT- MEDLINE
DCOM- 20150512
LR  - 20140916
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 969
DP  - 2014 Oct 15
TI  - Determination of phenolic acids and flavonoids in raw propolis by 
      silica-supported ionic liquid-based matrix solid phase dispersion extraction high 
      performance liquid chromatography-diode array detection.
PG  - 205-12
LID - S1570-0232(14)00539-X [pii]
LID - 10.1016/j.jchromb.2014.08.022 [doi]
AB  - The silica-supported ionic liquid (S-SIL) was prepared by impregnation and used 
      as the dispersion adsorbent of matrix solid phase dispersion (MSPD) for the 
      simultaneous extraction of eight phenolic acids and flavonoids, including caffeic 
      acid, ferulic acid, morin, luteolin, quercetin, apigenin, chrysin, and 
      kaempferide in raw propolis. High performance liquid chromatography with a Zorbax 
      SB-C18 column (150mmx4.6mm, 3.5mum) was used for separation of the analytes. The 
      mobile phase consisted of 0.2% phosphoric acid aqueous solution and acetonitrile 
      and the flow rate of the mobile phase was 0.5mL/min. The experimental conditions 
      for silica-supported ionic liquid-based matrix solid phase dispersion 
      (S-SIL-based MSPD) were optimized. S-SIL containing 10% [C6MIM]Cl was used as 
      dispersant, 20mL of n-hexane as washing solvent and 15mL of methanol as elution 
      solvent. The ratio of S-SIL to sample was selected to be 4:1. The standard curves 
      showed good linear relationship (r>0.9995). The limits of detection and 
      quantification were in the range of 5.8-22.2ngmL(-1) and 19.2-74.0ngmL(-1), 
      respectively. The relative standard deviations (RSDs) of intra-day and inter-day 
      determination were lower than 8.80% and 11.19%, respectively. The recoveries were 
      between 65.51% and 92.32% with RSDs lower than 8.95%. Compared with 
      ultrasound-assisted extraction (UAE) and soxhlet extraction, the present method 
      consumed less sample, organic solvent, and extraction time, although the 
      extraction yields obtained by S-SIL-based MSPD are slightly lower than those 
      obtained by UAE.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Wang, Zhibing
AU  - Wang Z
AD  - College of Chemistry and Life Science, Changchun University of Technology, 
      Changchun 130012, China; College of Chemistry, Jilin University, Changchun 
      130012, China.
FAU - Sun, Rui
AU  - Sun R
AD  - College of Chemistry and Life Science, Changchun University of Technology, 
      Changchun 130012, China; School of Food Science and Technology, Dalian 
      Polytechnic University, Dalian 116034, China.
FAU - Wang, Yuanpeng
AU  - Wang Y
AD  - College of Chemistry, Jilin University, Changchun 130012, China.
FAU - Li, Na
AU  - Li N
AD  - College of Chemistry, Jilin University, Changchun 130012, China.
FAU - Lei, Lei
AU  - Lei L
AD  - College of Chemistry, Jilin University, Changchun 130012, China.
FAU - Yang, Xiao
AU  - Yang X
AD  - College of Chemistry, Jilin University, Changchun 130012, China.
FAU - Yu, Aimin
AU  - Yu A
AD  - College of Chemistry, Jilin University, Changchun 130012, China.
FAU - Qiu, Fangping
AU  - Qiu F
AD  - College of Chemistry and Life Science, Changchun University of Technology, 
      Changchun 130012, China. Electronic address: analchem@jlu.edu.cn.
FAU - Zhang, Hanqi
AU  - Zhang H
AD  - College of Chemistry, Jilin University, Changchun 130012, China.
LA  - eng
PT  - Journal Article
DEP - 20140827
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Flavonoids)
RN  - 0 (Ionic Liquids)
RN  - 0 (Phenols)
RN  - 9009-62-5 (Propolis)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Flavonoids/*analysis/chemistry/isolation & purification
MH  - Ionic Liquids/chemistry
MH  - Phenols/*analysis/chemistry/isolation & purification
MH  - Propolis/*chemistry
MH  - Reproducibility of Results
MH  - Solid Phase Extraction/*methods
OTO - NOTNLM
OT  - High-performance liquid chromatography (HPLC)
OT  - Phenolic acids and flavonoids
OT  - Raw propolis
OT  - Silica-supported ionic liquid-based matrix solid phase dispersion (S-SIL-based 
      MSPD)
EDAT- 2014/09/06 06:00
MHDA- 2015/05/13 06:00
CRDT- 2014/09/06 06:00
PHST- 2014/06/19 00:00 [received]
PHST- 2014/08/14 00:00 [revised]
PHST- 2014/08/17 00:00 [accepted]
PHST- 2014/09/06 06:00 [entrez]
PHST- 2014/09/06 06:00 [pubmed]
PHST- 2015/05/13 06:00 [medline]
AID - S1570-0232(14)00539-X [pii]
AID - 10.1016/j.jchromb.2014.08.022 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Oct 15;969:205-12. doi: 
      10.1016/j.jchromb.2014.08.022. Epub 2014 Aug 27.