PMID- 25190302
OWN - NLM
STAT- MEDLINE
DCOM- 20150703
LR  - 20141121
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 174
IP  - 7
DP  - 2014 Dec
TI  - Microbially induced calcite precipitation-based sequestration of strontium by 
      Sporosarcina pasteurii WJ-2.
PG  - 2482-91
LID - 10.1007/s12010-014-1196-4 [doi]
AB  - Contamination by radioactive strontium ((90)Sr) is a significant environmental 
      problem. Ureolytically driven calcium carbonate precipitation has been proposed 
      for use in geotechnical engineering for soil remediation applications. In this 
      study, 68 ureolytic bacterial strains were newly isolated from various 
      environments. Of these, 19 strains were selected based on ureolytic activity 
      shown when cultured on urea agar plates and identified through 16S rRNA gene 
      sequencing. From these selected strains, Sporosarcina pasteurii WJ-2 (WJ-2) was 
      selected for subsequent study. A simple method was developed to determine the 
      effectiveness of microbially induced calcite precipitation (MICP). Unlike any 
      other methods, it does not require advanced skills and sophisticated tools. Using 
      this method, we were able to determine the ability of the bioconsolidated sand to 
      retard the flow of crystal violet through the 25-mL column. Also, MICP by WJ-2 
      was evaluated for its potential to counteract Sr contamination in column 
      experiments using natural sand. WJ-2-induced precipitation led to successful 
      sequestration of approximately 80 % of the Sr from the soluble fraction of the 
      sand. The utility of MICP in bioremediation was further confirmed through X-ray 
      diffraction, scanning electron microscopy, and inductively coupled plasma mass 
      spectrometry.
FAU - Kang, Chang-Ho
AU  - Kang CH
AD  - Department of Biological Engineering, Inha University, Yonghyun-dong 253, Nam-gu, 
      Incheon, 402-751, South Korea.
FAU - Choi, Jae-Ho
AU  - Choi JH
FAU - Noh, JunGu
AU  - Noh J
FAU - Kwak, Dae Young
AU  - Kwak DY
FAU - Han, Sang-Hyun
AU  - Han SH
FAU - So, Jae-Seong
AU  - So JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140905
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Soil Pollutants, Radioactive)
RN  - 0 (Strontium Isotopes)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - YZS2RPE8LE (Strontium)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Calcium Carbonate/*metabolism
MH  - Soil Pollutants, Radioactive/*metabolism
MH  - Sporosarcina/*growth & development
MH  - Strontium/*metabolism
MH  - Strontium Isotopes/metabolism
EDAT- 2014/09/06 06:00
MHDA- 2015/07/04 06:00
CRDT- 2014/09/06 06:00
PHST- 2013/07/17 00:00 [received]
PHST- 2013/10/29 00:00 [accepted]
PHST- 2014/09/06 06:00 [entrez]
PHST- 2014/09/06 06:00 [pubmed]
PHST- 2015/07/04 06:00 [medline]
AID - 10.1007/s12010-014-1196-4 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2014 Dec;174(7):2482-91. doi: 10.1007/s12010-014-1196-4. 
      Epub 2014 Sep 5.