PMID- 25191744
OWN - NLM
STAT- MEDLINE
DCOM- 20160317
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 9
DP  - 2014
TI  - Alterations in gene array patterns in dendritic cells from aged humans.
PG  - e106471
LID - 10.1371/journal.pone.0106471 [doi]
LID - e106471
AB  - Dendritic cells (DCs) are major antigen-presenting cells that play a key role in 
      initiating and regulating innate and adaptive immune responses. DCs are critical 
      mediators of tolerance and immunity. The functional properties of DCs decline 
      with age. The purpose of this study was to define the age-associated molecular 
      changes in DCs by gene array analysis using Affymatrix GeneChips. The expression 
      levels of a total of 260 genes (1.8%) were significantly different (144 
      down-regulated and 116 upregulated) in monocyte-derived DCs (MoDCs) from aged 
      compared to young human donors. Of the 260 differentially expressed genes, 24% 
      were down-regulated by more than 3-fold, suggesting that a large reduction in 
      expression occurred for a notable number of genes in the aged. Our results 
      suggest that the genes involved in immune response to pathogens, cell migration 
      and T cell priming display significant age-related changes. Furthermore, 
      downregulated genes involved in cell cycle arrest and DNA replication may play a 
      critical role in aging-associated genetic instability. These changes in gene 
      expression provide molecular based evidence for age-associated functional 
      abnormalities in human DCs that may be responsible for the defects in adaptive 
      immunity observed in the elderly.
FAU - Cao, Jia-ning
AU  - Cao JN
AD  - Division of Basic and Clinical Immunology, Department of Medicine, University of 
      California Irvine, Irvine, California, United States of America.
FAU - Agrawal, Anshu
AU  - Agrawal A
AD  - Division of Basic and Clinical Immunology, Department of Medicine, University of 
      California Irvine, Irvine, California, United States of America.
FAU - Sharman, Edward
AU  - Sharman E
AD  - Department of Neurology, University of California Irvine, Irvine, California, 
      United States of America.
FAU - Jia, Zhenyu
AU  - Jia Z
AD  - Department of Statistics, University of Akron, Akron, Ohio, United States of 
      America; Department of Family and Community Medicine, Northeast Ohio Medical 
      University, Rootstown, Ohio, United States of America; Pathology & Laboratory 
      Medicine, University of California Irvine, Irvine, California, United States of 
      America.
FAU - Gupta, Sudhir
AU  - Gupta S
AD  - Division of Basic and Clinical Immunology, Department of Medicine, University of 
      California Irvine, Irvine, California, United States of America.
LA  - eng
PT  - Journal Article
DEP - 20140905
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Adaptive Immunity/genetics
MH  - Adult
MH  - Age Factors
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigen-Presenting Cells/immunology/metabolism
MH  - Cluster Analysis
MH  - Dendritic Cells/immunology/*metabolism
MH  - Female
MH  - G1 Phase
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Healthy Volunteers
MH  - Humans
MH  - Immunity, Innate/genetics
MH  - Male
MH  - *Transcriptome
MH  - Young Adult
PMC - PMC4156347
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/09/06 06:00
MHDA- 2016/03/18 06:00
PMCR- 2014/09/05
CRDT- 2014/09/06 06:00
PHST- 2014/02/26 00:00 [received]
PHST- 2014/07/30 00:00 [accepted]
PHST- 2014/09/06 06:00 [entrez]
PHST- 2014/09/06 06:00 [pubmed]
PHST- 2016/03/18 06:00 [medline]
PHST- 2014/09/05 00:00 [pmc-release]
AID - PONE-D-14-07345 [pii]
AID - 10.1371/journal.pone.0106471 [doi]
PST - epublish
SO  - PLoS One. 2014 Sep 5;9(9):e106471. doi: 10.1371/journal.pone.0106471. eCollection 
      2014.