PMID- 25191937
OWN - NLM
STAT- MEDLINE
DCOM- 20160317
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 9
DP  - 2014
TI  - Biopsy of human morula-stage embryos: outcome of 215 IVF/ICSI cycles with PGS.
PG  - e106433
LID - 10.1371/journal.pone.0106433 [doi]
LID - e106433
AB  - Preimplantation genetic diagnosis (PGD) is commonly performed on biopsies from 
      6-8-cell-stage embryos or blastocyst trophectoderm obtained on day 3 or 5, 
      respectively. Day 4 human embryos at the morula stage were successfully biopsied. 
      Biopsy was performed on 709 morulae from 215 ICSI cycles with preimplantation 
      genetic screening (PGS), and 3-7 cells were obtained from each embryo. The most 
      common vital aneuploidies (chromosomes X/Y, 21) were screened by fluorescence in 
      situ hybridization (FISH). No aneuploidy was observed in 72.7% of embryos, 91% of 
      those developed to blastocysts. Embryos were transferred on days 5-6. Clinical 
      pregnancy was obtained in 32.8% of cases, and 60 babies were born. Patients who 
      underwent ICSI/PGS treatment were compared with those who underwent standard ICSI 
      treatment by examining the percentage of blastocysts, pregnancy rate, gestational 
      length, birth height and weight. No significant differences in these parameters 
      were observed between the groups. Day 4 biopsy procedure does not adversely 
      affect embryo development in vitro or in vivo. The increased number of cells 
      obtained by biopsy of morulae might facilitate diagnostic screening. There is 
      enough time after biopsy to obtain PGD results for embryo transfer on day 5-6 in 
      the current IVF cycle.
FAU - Zakharova, Elena E
AU  - Zakharova EE
AD  - Center for Reproductive Medicine MAMA, Moscow, Russian Federation.
FAU - Zaletova, Victoria V
AU  - Zaletova VV
AD  - Center for Reproductive Medicine MAMA, Moscow, Russian Federation.
FAU - Krivokharchenko, Alexander S
AU  - Krivokharchenko AS
AD  - Center for Reproductive Medicine MAMA, Moscow, Russian Federation.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140905
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Adult
MH  - Biopsy
MH  - Embryo Transfer
MH  - Female
MH  - Fertilization in Vitro
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Morula/*cytology
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - Pregnancy Rate
MH  - Preimplantation Diagnosis
MH  - Sperm Injections, Intracytoplasmic
PMC - PMC4156362
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/09/06 06:00
MHDA- 2016/03/18 06:00
PMCR- 2014/09/05
CRDT- 2014/09/06 06:00
PHST- 2014/05/20 00:00 [received]
PHST- 2014/07/29 00:00 [accepted]
PHST- 2014/09/06 06:00 [entrez]
PHST- 2014/09/06 06:00 [pubmed]
PHST- 2016/03/18 06:00 [medline]
PHST- 2014/09/05 00:00 [pmc-release]
AID - PONE-D-14-22425 [pii]
AID - 10.1371/journal.pone.0106433 [doi]
PST - epublish
SO  - PLoS One. 2014 Sep 5;9(9):e106433. doi: 10.1371/journal.pone.0106433. eCollection 
      2014.