PMID- 25193075
OWN - NLM
STAT- MEDLINE
DCOM- 20150326
LR  - 20201023
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 331
IP  - 1
DP  - 2015 Feb 1
TI  - Intrinsic mechanisms underlying the neurotrophic activity of adipose derived stem 
      cells.
PG  - 142-151
LID - S0014-4827(14)00372-3 [pii]
LID - 10.1016/j.yexcr.2014.08.034 [doi]
AB  - Adipose derived stem cells (ADSC) can be differentiated into Schwann cell-like 
      cells which enhance nerve function and regeneration. However, the signalling 
      mechanisms underlying the neurotrophic potential of ADSC remain largely unknown. 
      In this study, we hypothesised that ADSC, upon stimulation with a combination of 
      growth factors, could rapidly produce brain derived neurotrophic factor (BDNF) 
      with a similar molecular mechanism to that functioning in the nervous system. 
      Within 48 h of stimulation, ADSC demonstrated potent neurotrophic effects on 
      dorsal root ganglion neurons, at a magnitude equivalent to that of the longer 
      term differentiated Schwann cell-like cells. Stimulated ADSC showed rapid 
      up-regulation of the neuronal activity dependent promoter BDNF exon IV along with 
      an augmented expression of full length protein encoding BDNF exon IX. BDNF 
      protein was secreted at a concentration similar to that produced by 
      differentiated Schwann cell-like cells. Stimulation also activated the BDNF 
      expression gating transcription factor, cAMP responsive element binding (CREB) 
      protein. However, blocking phosphorylation of CREB with the protein kinase A 
      small molecule inhibitor H89 did not suppress secretion of BDNF protein. These 
      results suggest rapid BDNF production in ADSC is mediated through multiple 
      compensatory pathways independent of, or in addition to, the CREB neuronal 
      activation cascade.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Tse, Kai-Hei
AU  - Tse KH
AD  - Department of Integrative Medical Biology, Section of Anatomy, Umea University, 
      SE-901 87 Umea, Sweden.
FAU - Novikov, Lev N
AU  - Novikov LN
AD  - Department of Integrative Medical Biology, Section of Anatomy, Umea University, 
      SE-901 87 Umea, Sweden.
FAU - Wiberg, Mikael
AU  - Wiberg M
AD  - Department of Integrative Medical Biology, Section of Anatomy, Umea University, 
      SE-901 87 Umea, Sweden; Department of Surgical and Perioperative Sciences, 
      Section of Hand & Plastic Surgery, Umea University, Sweden.
FAU - Kingham, Paul J
AU  - Kingham PJ
AD  - Department of Integrative Medical Biology, Section of Anatomy, Umea University, 
      SE-901 87 Umea, Sweden. Electronic address: paul.kingham@umu.se.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140901
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adipocytes/cytology/*metabolism
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Calcium/metabolism
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Ganglia, Spinal/cytology/*metabolism
MH  - Immunoenzyme Techniques
MH  - Nerve Growth Factors/genetics/metabolism
MH  - Neurites/*metabolism
MH  - Neurons/cytology/*metabolism
MH  - Phenotype
MH  - Phosphorylation
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Inbred F344
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Schwann Cells/cytology/*metabolism
MH  - Signal Transduction
MH  - Stem Cells/cytology/*metabolism
OTO - NOTNLM
OT  - Brain derived neurotrophic factor
OT  - Differentiation
OT  - Neurite
OT  - Regeneration
OT  - Stem cells
EDAT- 2014/09/07 06:00
MHDA- 2015/03/27 06:00
CRDT- 2014/09/07 06:00
PHST- 2014/07/20 00:00 [received]
PHST- 2014/08/24 00:00 [accepted]
PHST- 2014/09/07 06:00 [entrez]
PHST- 2014/09/07 06:00 [pubmed]
PHST- 2015/03/27 06:00 [medline]
AID - S0014-4827(14)00372-3 [pii]
AID - 10.1016/j.yexcr.2014.08.034 [doi]
PST - ppublish
SO  - Exp Cell Res. 2015 Feb 1;331(1):142-151. doi: 10.1016/j.yexcr.2014.08.034. Epub 
      2014 Sep 1.