PMID- 25193115
OWN - NLM
STAT- MEDLINE
DCOM- 20150806
LR  - 20201209
IS  - 1873-3913 (Electronic)
IS  - 0898-6568 (Linking)
VI  - 26
IP  - 12
DP  - 2014 Dec
TI  - Role of NSD1 in H2O2-induced GSTM3 suppression.
PG  - 2757-64
LID - S0898-6568(14)00299-X [pii]
LID - 10.1016/j.cellsig.2014.08.026 [doi]
AB  - Nuclear receptor-binding SET domain-containing protein 1 (NSD1) has been proved 
      to act as a histone methyltransferase and a transcription co-factor to regulate 
      gene expression. However, the role of NSD1 in oxidative stress remains poorly 
      understood. In the present study, we focused on the NSD1 regulation of 
      antioxidant enzyme gene glutathione S-transferase M3 (GSTM3) expression in 
      response to oxidative stress. H2O2 treatment caused the decrease of both NSD1 and 
      GSTM3 expression, and the depletion of NSD1 expression by specific siRNA reversed 
      the H2O2-reduced GSTM3 expression. Furthermore, we investigated NSD1 modulating 
      the transcription of GSTM3 promoter with -63A/C polymorphism closed to TATA box 
      in response to H2O2 by luciferase and in vitro or in vivo DNA-protein binding 
      assays. The promoter activity of GSTM3 with -63A was higher than -63C, and was 
      increased or decreased by the overexpression or depletion of NSD1, but -63C was 
      not influenced. H2O2 repressed the promoter activity of GSTM3 with -63A more than 
      -63C, and the depletion of NSD1 expression weakened H2O2 inhibition on the -63A 
      promoter, but augmented H2O2 inhibition on the -63C promoter. In addition, NSD1 
      interacted with RNAPII and bound to GSTM3 -63A/C TATA box, with higher binding 
      affinity to -63A than to -63C. These data indicated that NSD1 implicated in 
      H2O2-induced oxidative stress, and H2O2-induced NSD1 suppression resulted in the 
      decrease of GSTM3 expression through the -63A/C TATA box.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Chu, Guoming
AU  - Chu G
AD  - Department of Clinical Genetics, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning, China; Department of Medical Genetics, China Medical 
      University, Shenyang, Liaoning, China.
FAU - Li, Yinghui
AU  - Li Y
AD  - Department of Medical Genetics, China Medical University, Shenyang, Liaoning, 
      China.
FAU - Dong, Xiaolong
AU  - Dong X
AD  - Department of Clinical Genetics, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning, China; Department of Medical Genetics, China Medical 
      University, Shenyang, Liaoning, China.
FAU - Liu, Jian
AU  - Liu J
AD  - Department of Medical Genetics, China Medical University, Shenyang, Liaoning, 
      China.
FAU - Zhao, Yanyan
AU  - Zhao Y
AD  - Department of Clinical Genetics, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning, China; Department of Medical Genetics, China Medical 
      University, Shenyang, Liaoning, China. Electronic address: 
      yyzhao@sj-hospital.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140902
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Antioxidants)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Nuclear Proteins)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 2.1.1.- (Histone Methyltransferases)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
RN  - EC 2.1.1.43 (NSD1 protein, human)
RN  - EC 2.5.1.18 (GSTM3 protein, human)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Antioxidants/metabolism
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Glutathione Transferase/*genetics/*metabolism
MH  - HEK293 Cells
MH  - Histone Methyltransferases
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - Hydrogen Peroxide/*pharmacology
MH  - Intracellular Signaling Peptides and Proteins/*genetics/*metabolism
MH  - Nuclear Proteins/*genetics/*metabolism
MH  - Oxidative Stress/genetics
MH  - Polymorphism, Genetic/genetics
MH  - TATA Box/genetics
MH  - Transcription, Genetic/genetics
OTO - NOTNLM
OT  - Glutathione S-transferase M3
OT  - Hydrogen peroxide
OT  - Nuclear receptor-binding SET domain-containing protein 1
OT  - Oxidative stress
OT  - Polymorphism
OT  - RNA polymerase II
EDAT- 2014/09/07 06:00
MHDA- 2015/08/08 06:00
CRDT- 2014/09/07 06:00
PHST- 2014/05/14 00:00 [received]
PHST- 2014/08/06 00:00 [revised]
PHST- 2014/08/26 00:00 [accepted]
PHST- 2014/09/07 06:00 [entrez]
PHST- 2014/09/07 06:00 [pubmed]
PHST- 2015/08/08 06:00 [medline]
AID - S0898-6568(14)00299-X [pii]
AID - 10.1016/j.cellsig.2014.08.026 [doi]
PST - ppublish
SO  - Cell Signal. 2014 Dec;26(12):2757-64. doi: 10.1016/j.cellsig.2014.08.026. Epub 
      2014 Sep 2.