PMID- 25193331
OWN - NLM
STAT- MEDLINE
DCOM- 20150429
LR  - 20181202
IS  - 1095-6840 (Electronic)
IS  - 0016-6480 (Linking)
VI  - 208
DP  - 2014 Nov 1
TI  - Age-dependent effect of obestatin on intestinal contractility in Wistar rats.
PG  - 109-15
LID - S0016-6480(14)00341-4 [pii]
LID - 10.1016/j.ygcen.2014.08.015 [doi]
AB  - Obestatin is a 23-amino acid peptide encoded by the ghrelin gene. We have 
      investigated the effect of obestatin on intestinal contractility in rats ranging 
      from the suckling period till adolescence. Duodenal and middle jejunum 
      whole-thickness preparations from neonatal and adult rats were studied in an 
      organ bath, for isometric recording under treatment with obestatin (1mumolL(-1)) 
      in the presence of acetylocholine (ACh), atropine and tetradotoxin (TTX). Both 
      the EFS and ACh-stimulated contractile response, as well as spontaneous 
      contractile activity is age-dependent and specific for the segment of jejunum. 
      Except for the middle jejunum of 7day old rats, treatment with obestatin caused a 
      significant TTX-sensitive increase in the amplitude of EFS-stimulated 
      off-contraction of both intestinal segments studied. Following injection of 
      obestatin, the amplitude of spontaneous contraction in the duodenum increased in 
      7day old rats. In the middle jejunum, treatment with obestatin significantly 
      increased both the amplitude and frequency of spontaneous contraction in rats 
      till the 28th day of life, whereas in adult rats the observed effect of obestatin 
      was the opposite (P<0.001 and P<0.0001, respectively). The effects of treatment 
      with obestatin on stimulation with increasing doses of ACh were only observed in 
      the preparations from suckling rats. ACh-stimulated contractility in the duodenum 
      was decreased while in the middle jejunum the observed effect was opposite. These 
      results indicate the importance of peripheral obestatin in the cholinergic 
      control of intestinal contractility in both neonatal and adult rats.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Slupecka, M
AU  - Slupecka M
AD  - Department of Endocrinology, The Kielanowski Institute of Animal Physiology and 
      Nutrition, Polish Academy of Sciences, Jablonna, Poland. Electronic address: 
      m.slupecka@ifzz.pan.pl.
FAU - Pierzynowski, S G
AU  - Pierzynowski SG
AD  - Department of Biology, Lund University, Lund, Sweden; Department of Medical 
      Biology, Institute of Agricultural Medicine, Lublin, Poland.
FAU - Kuwahara, A
AU  - Kuwahara A
AD  - Laboratory of Physiology, Institute for Environmental Sciences & Graduate School 
      of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka, 
      Japan.
FAU - Kato, I
AU  - Kato I
AD  - Department of Bioorganic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku 
      University, Kanazawa, Japan.
FAU - Wolinski, J
AU  - Wolinski J
AD  - Department of Endocrinology, The Kielanowski Institute of Animal Physiology and 
      Nutrition, Polish Academy of Sciences, Jablonna, Poland.
LA  - eng
PT  - Journal Article
DEP - 20140902
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (Ghrelin)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Aging/*physiology
MH  - Animals
MH  - Duodenum/drug effects/physiology
MH  - Electric Stimulation
MH  - Ghrelin/*pharmacology
MH  - In Vitro Techniques
MH  - Intestines/drug effects/*physiology
MH  - Jejunum/drug effects/physiology
MH  - Male
MH  - Muscle Contraction/*drug effects
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Contractility
OT  - Intestine
OT  - Neonates
OT  - Obestatin
EDAT- 2014/09/07 06:00
MHDA- 2015/04/30 06:00
CRDT- 2014/09/07 06:00
PHST- 2014/03/17 00:00 [received]
PHST- 2014/05/28 00:00 [revised]
PHST- 2014/08/10 00:00 [accepted]
PHST- 2014/09/07 06:00 [entrez]
PHST- 2014/09/07 06:00 [pubmed]
PHST- 2015/04/30 06:00 [medline]
AID - S0016-6480(14)00341-4 [pii]
AID - 10.1016/j.ygcen.2014.08.015 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2014 Nov 1;208:109-15. doi: 10.1016/j.ygcen.2014.08.015. 
      Epub 2014 Sep 2.