PMID- 25193850
OWN - NLM
STAT- MEDLINE
DCOM- 20141125
LR  - 20181202
IS  - 1873-0183 (Electronic)
IS  - 1568-9972 (Linking)
VI  - 13
IP  - 10
DP  - 2014 Oct
TI  - Cortactin autoantibodies in myasthenia gravis.
PG  - 1003-7
LID - S1568-9972(14)00183-9 [pii]
LID - 10.1016/j.autrev.2014.08.039 [doi]
AB  - Myasthenia gravis (MG) is an autoimmune disease characterized by muscle weakness, 
      fatigability, and autoantibodies against protein antigens of the muscle endplate. 
      Antibodies against acetylcholine receptor (AChR), and less frequently against 
      muscle-Specific Kinase (MuSK) or lipoprotein related protein 4 (LRP4) occur in 
      patients with seropositive MG (SPMG). However, about 10% of patients do not have 
      detectable autoantibodies despite evidence suggesting that the disorder is immune 
      mediated; this disorder is known as seronegative MG (SNMG). Using a protein array 
      approach we identified cortactin (a protein that acts downstream from agrin/MuSK 
      promoting AChR clustering) as potential new target antigen in SNMG. We set up an 
      ELISA assay and screened sera from patients with SPMG, SNMG, other autoimmune 
      diseases and controls. Results were validated by immunoblot. We found that 19.7% 
      of patients with SNMG had antibodies against cortactin whereas only 4.8% of 
      patients with SPMG were positive. Cortactin antibodies were also found in 12.5% 
      of patients with other autoimmune disorders but only in 5.2% of healthy controls. 
      We conclude that the finding of cortactin antibodies in patients with SNMG, 
      suggests an underlying autoimmune mechanism, supporting the use of immune 
      therapy.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Gallardo, Eduard
AU  - Gallardo E
AD  - Neuromuscular Diseases Unit, Hospital de la Santa Creu i Sant Pau, Universitat 
      Autonoma de Barcelona, Barcelona, Spain.
FAU - Martinez-Hernandez, Eugenia
AU  - Martinez-Hernandez E
AD  - Neuromuscular Diseases Unit, Hospital de la Santa Creu i Sant Pau, Universitat 
      Autonoma de Barcelona, Barcelona, Spain.
FAU - Titulaer, Maarten J
AU  - Titulaer MJ
AD  - Department of Neurology, Erasmus Medical Center, Rotterdam, The Netherlands.
FAU - Huijbers, Maartje G
AU  - Huijbers MG
AD  - Department of Neurology, Leiden University Medical Center, Leiden, The 
      Netherlands.
FAU - Martinez, Maria Angeles
AU  - Martinez MA
AD  - Department of Immunology, Hospital de la Santa Creu i Sant Pau, Universitat 
      Autonoma de Barcelona, Barcelona, Spain.
FAU - Ramos, Alba
AU  - Ramos A
AD  - Neuromuscular Diseases Unit, Hospital de la Santa Creu i Sant Pau, Universitat 
      Autonoma de Barcelona, Barcelona, Spain.
FAU - Querol, Luis
AU  - Querol L
AD  - Neuromuscular Diseases Unit, Hospital de la Santa Creu i Sant Pau, Universitat 
      Autonoma de Barcelona, Barcelona, Spain.
FAU - Diaz-Manera, Jordi
AU  - Diaz-Manera J
AD  - Neuromuscular Diseases Unit, Hospital de la Santa Creu i Sant Pau, Universitat 
      Autonoma de Barcelona, Barcelona, Spain.
FAU - Rojas-Garcia, Ricard
AU  - Rojas-Garcia R
AD  - Neuromuscular Diseases Unit, Hospital de la Santa Creu i Sant Pau, Universitat 
      Autonoma de Barcelona, Barcelona, Spain.
FAU - Hayworth, Christopher R
AU  - Hayworth CR
AD  - Department of Neuroscience, University of Pennsylvania, PA, USA.
FAU - Verschuuren, Jan J
AU  - Verschuuren JJ
AD  - Department of Neurology, Leiden University Medical Center, Leiden, The 
      Netherlands.
FAU - Balice-Gordon, Rita
AU  - Balice-Gordon R
AD  - Department of Neuroscience, University of Pennsylvania, PA, USA.
FAU - Dalmau, Josep
AU  - Dalmau J
AD  - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Hospital 
      Clinic, Universitat de Barcelona, Barcelona, Spain; Department of Neurology, 
      University of Pennsylvania, Philadelphia, PA, USA; Institucio Catalana de Recerca 
      i Estudis Avancats (ICREA), Barcelona, Spain.
FAU - Illa, Isabel
AU  - Illa I
AD  - Neuromuscular Diseases Unit, Hospital de la Santa Creu i Sant Pau, Universitat 
      Autonoma de Barcelona, Barcelona, Spain. Electronic address: iilla@santpau.cat.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140903
PL  - Netherlands
TA  - Autoimmun Rev
JT  - Autoimmunity reviews
JID - 101128967
RN  - 0 (Autoantibodies)
RN  - 0 (CTTN protein, human)
RN  - 0 (Cortactin)
RN  - 0 (Receptors, Cholinergic)
SB  - IM
MH  - Autoantibodies/*analysis/immunology
MH  - Cortactin/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Male
MH  - Myasthenia Gravis/*immunology
MH  - Protein Array Analysis
MH  - Receptors, Cholinergic/immunology
OTO - NOTNLM
OT  - Acetylcholine receptor
OT  - Cortactin
OT  - Musk
OT  - Myasthenia
EDAT- 2014/09/07 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/09/07 06:00
PHST- 2014/06/19 00:00 [received]
PHST- 2014/06/25 00:00 [accepted]
PHST- 2014/09/07 06:00 [entrez]
PHST- 2014/09/07 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S1568-9972(14)00183-9 [pii]
AID - 10.1016/j.autrev.2014.08.039 [doi]
PST - ppublish
SO  - Autoimmun Rev. 2014 Oct;13(10):1003-7. doi: 10.1016/j.autrev.2014.08.039. Epub 
      2014 Sep 3.