PMID- 25194809
OWN - NLM
STAT- MEDLINE
DCOM- 20150225
LR  - 20140929
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 452
IP  - 3
DP  - 2014 Sep 26
TI  - Hyperhomocysteinemia-induced oxidative stress differentially alters proteasome 
      composition and activities in heart and aorta.
PG  - 740-5
LID - S0006-291X(14)01582-4 [pii]
LID - 10.1016/j.bbrc.2014.08.141 [doi]
AB  - BACKGROUND AND AIMS: Hyperhomocysteinemia (HHcy) is associated with 
      cardiovascular diseases and is thought to induce endogenous oxidative stress and 
      causes many cellular damages. Proteasome that degrades oxidized and ubiquitinated 
      proteins can regulate the cellular response to oxidative stress. We aimed to 
      investigate whether hyperhomocysteinemia induces oxidative stress and alters 
      proteasome function and composition in heart and aorta tissues of rat. METHODS 
      AND RESULTS: To create hyperhomocysteinemia, male Wistar rats (Pasteur 
      Institute-Algiers) were received daily intraperitoneal injections of 
      dl-homocysteine (0.6-1.2muM/g body weight) for 3weeks. Biomarkers of oxidative 
      stress (malondialdehyde (MDA), protein carbonyl (PC), superoxide dismutase (SOD) 
      and catalase (CAT)) were first measured by biochemical methods and tissue damages 
      by histological sections. Proteasome activities were quantitated using 
      fluorogenic synthetic peptides; ubiquitinated proteins and proteasome subunits 
      expression were then evaluated by SDS PAGE and Western blot analysis. We showed 
      increased MDA and PC but decreased SOD and CAT levels both in plasma, heart and 
      aorta accompanied by histological changes. A significant decrease of proteasome 
      activities was observed in heart, whereas proteasome activity was not affected in 
      aorta. However proteasome composition was altered in both tissues, as the 
      accumulation of ubiquitinated proteins. CONCLUSION: Data demonstrated an 
      alteration of the ubiquitin-proteasome system in hyperhomocysteinemia as a result 
      of accumulating oxidized and ubiquitinated proteins in response to oxidative 
      stress. Further studies must be conducted to better understanding mechanisms 
      responsible of proteasome alterations in hyperhomocysteinemia.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Derouiche, Faouzia
AU  - Derouiche F
AD  - Laboratory of Microbiological Engineering and Application (team of cellular 
      physiology and molecular biology), Constantine University 1, Constantine, 
      Algeria; Khenchela University, Khenchela, Algeria.
FAU - Bole-Feysot, Christine
AU  - Bole-Feysot C
AD  - INSERM Unit 1073, Rouen, France; Institute for Research and Innovation in 
      Biomedicine, Rouen University, Rouen, France.
FAU - Naimi, Dalila
AU  - Naimi D
AD  - Laboratory of Microbiological Engineering and Application (team of cellular 
      physiology and molecular biology), Constantine University 1, Constantine, 
      Algeria.
FAU - Coeffier, Moise
AU  - Coeffier M
AD  - INSERM Unit 1073, Rouen, France; Institute for Research and Innovation in 
      Biomedicine, Rouen University, Rouen, France; Rouen University Hospital, 
      Nutrition Unit, Rouen, France. Electronic address: moise.coeffier@chu-rouen.fr.
LA  - eng
PT  - Journal Article
DEP - 20140904
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Ubiquitinated Proteins)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Aorta/*metabolism/pathology
MH  - Catalase/genetics/metabolism
MH  - Gene Expression Regulation
MH  - Glutathione/metabolism
MH  - Glutathione Peroxidase/genetics/metabolism
MH  - Homocysteine/administration & dosage
MH  - Hyperhomocysteinemia/chemically induced/genetics/*metabolism/pathology
MH  - Injections, Intraperitoneal
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Myocardium/*metabolism/pathology
MH  - Oxidation-Reduction
MH  - Oxidative Stress
MH  - Proteasome Endopeptidase Complex/chemistry/genetics/*metabolism
MH  - Protein Carbonylation
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction
MH  - Superoxide Dismutase/genetics/metabolism
MH  - Ubiquitinated Proteins/genetics/metabolism
MH  - Ubiquitination
OTO - NOTNLM
OT  - Aorta
OT  - Heart
OT  - Homocysteine
OT  - Oxidative stress
OT  - Proteasome
OT  - ROS
EDAT- 2014/09/10 06:00
MHDA- 2015/02/26 06:00
CRDT- 2014/09/08 06:00
PHST- 2014/08/19 00:00 [received]
PHST- 2014/08/26 00:00 [accepted]
PHST- 2014/09/08 06:00 [entrez]
PHST- 2014/09/10 06:00 [pubmed]
PHST- 2015/02/26 06:00 [medline]
AID - S0006-291X(14)01582-4 [pii]
AID - 10.1016/j.bbrc.2014.08.141 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2014 Sep 26;452(3):740-5. doi: 
      10.1016/j.bbrc.2014.08.141. Epub 2014 Sep 4.