PMID- 25194984
OWN - NLM
STAT- MEDLINE
DCOM- 20150728
LR  - 20171116
IS  - 1638-6183 (Electronic)
IS  - 0300-9084 (Linking)
VI  - 106
DP  - 2014 Nov
TI  - MicroRNA-152 modulates the canonical Wnt pathway activation by targeting DNA 
      methyltransferase 1 in arthritic rat model.
PG  - 149-56
LID - S0300-9084(14)00247-8 [pii]
LID - 10.1016/j.biochi.2014.08.016 [doi]
AB  - Rheumatoid arthritis (RA) is an autoimmune and progressive systemic disease of 
      unknown etiology. Research shows that fibroblast-like synoviocytes (FLS) 
      participate in the cartilage erosion, synovial hyperplasia, inflammatory cytokine 
      secretion and suggests that fibroblast-like synoviocytes (FLS) display a crucial 
      role in RA pathogenesis. Recent studies have suggested the role of the Wnt 
      signaling pathway in the pathogenesis of RA. In previous study, we identified 
      that increased methyl-CpG-binding protein 2 (MeCP2) reduced the secreted 
      frizzled-related protein 4 (SFRP4) expression in FLS in Arthritic rat model and 
      the DNA methyltransferase (DNMT) inhibitor 5-Aza-2'-deoxycytidine (5-azadC) could 
      induce the SFRP4 expression, indicating that DNMT has a key role in the 
      differential expression of SFRP4. MicroRNAs (MiRNAs), which are small non-coding 
      RNAs, are involved in diverse biological functions, regulation of gene 
      expression, pathogenesis of autoimmune disease and carcinogenesis. In light of 
      the directly down-regulation of miR-152 on DNMT1 expression by targeting the 3' 
      untranslated regions of its transcript in nickel sulfide (NiS)-transformed human 
      bronchial epithelial cells, we investigated whether miR-152 is aberrantly 
      expressed and targets DNMT1 in FLS in Arthritic rat model. Our results 
      demonstrated that the expression of miR-152 was specifically down-regulated in 
      Arthritic rat model, whereas up-regulation of miR-152 in FLS resulted in a marked 
      reduction of DNMT1 expression. Further experiments revealed that increased 
      miR-152 indirectly up-regulated the SFRP4 expression, a negative regulator of WNT 
      signaling pathway, by targeting the DNMT1. Moreover, activation of miR-152 
      expression in FLS could inhibit the canonical Wnt pathway activation and result 
      in a significant decrease of FLS proliferation. MiR-152 and DNA methylation may 
      provide molecular mechanisms for the activation of canonical Wnt pathway in RA. 
      Combination of miR-152 and DNMT1 may be a promising treatment strategy for RA 
      patients in which SFRP4 is inactivated.
CI  - Copyright (c) 2014 Elsevier B.V. and Societe francaise de biochimie et biologie 
      Moleculaire (SFBBM). All rights reserved.
FAU - Miao, Cheng-Gui
AU  - Miao CG
AD  - School of Food and Drug, Anhui Key Laboratory of Poultry Epidemic Prevention and 
      Surveillance, Anhui Science and Technology University, Bengbu 233100, China; 
      School of Pharmacy, Anhui Key Laboratory of Bioactivity of Natural Products, 
      Anhui Medical University, Hefei 230032, China.
FAU - Yang, Ying-Ying
AU  - Yang YY
AD  - School of Pharmacy, Anhui Key Laboratory of Bioactivity of Natural Products, 
      Anhui Medical University, Hefei 230032, China.
FAU - He, Xu
AU  - He X
AD  - School of Pharmacy, Anhui Key Laboratory of Bioactivity of Natural Products, 
      Anhui Medical University, Hefei 230032, China.
FAU - Huang, Cheng
AU  - Huang C
AD  - School of Pharmacy, Anhui Key Laboratory of Bioactivity of Natural Products, 
      Anhui Medical University, Hefei 230032, China.
FAU - Huang, Yan
AU  - Huang Y
AD  - School of Pharmacy, Anhui Key Laboratory of Bioactivity of Natural Products, 
      Anhui Medical University, Hefei 230032, China.
FAU - Qin, Dan
AU  - Qin D
AD  - School of Food and Drug, Anhui Key Laboratory of Poultry Epidemic Prevention and 
      Surveillance, Anhui Science and Technology University, Bengbu 233100, China.
FAU - Du, Chuan-Lai
AU  - Du CL
AD  - School of Food and Drug, Anhui Key Laboratory of Poultry Epidemic Prevention and 
      Surveillance, Anhui Science and Technology University, Bengbu 233100, China.
FAU - Li, Jun
AU  - Li J
AD  - School of Pharmacy, Anhui Key Laboratory of Bioactivity of Natural Products, 
      Anhui Medical University, Hefei 230032, China. Electronic address: 
      lijunamu@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140904
PL  - France
TA  - Biochimie
JT  - Biochimie
JID - 1264604
RN  - 0 (MIRN152 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Sfrp4 protein, rat)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
RN  - EC 2.1.1.37 (DNMT1 protein, human)
RN  - EC 2.1.1.37 (Dnmt1 protein, rat)
SB  - IM
MH  - Animals
MH  - Arthritis, Experimental/*genetics/metabolism
MH  - Cell Proliferation/genetics
MH  - Cells, Cultured
MH  - DNA (Cytosine-5-)-Methyltransferase 1
MH  - DNA (Cytosine-5-)-Methyltransferases/*genetics/metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Immunoblotting
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Proto-Oncogene Proteins/genetics/metabolism
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Synovial Membrane/cytology/metabolism
MH  - Wnt Signaling Pathway/*genetics
OTO - NOTNLM
OT  - Canonical Wnt pathway
OT  - DNA methyltransferase 1
OT  - MicroRNA-152
OT  - Rheumatoid arthritis
OT  - Secreted frizzled-related protein 4
EDAT- 2014/09/10 06:00
MHDA- 2015/07/29 06:00
CRDT- 2014/09/08 06:00
PHST- 2014/03/11 00:00 [received]
PHST- 2014/08/22 00:00 [accepted]
PHST- 2014/09/08 06:00 [entrez]
PHST- 2014/09/10 06:00 [pubmed]
PHST- 2015/07/29 06:00 [medline]
AID - S0300-9084(14)00247-8 [pii]
AID - 10.1016/j.biochi.2014.08.016 [doi]
PST - ppublish
SO  - Biochimie. 2014 Nov;106:149-56. doi: 10.1016/j.biochi.2014.08.016. Epub 2014 Sep 
      4.