PMID- 25195272
OWN - NLM
STAT- MEDLINE
DCOM- 20150113
LR  - 20140908
IS  - 2096-7993 (Print)
IS  - 2096-7993 (Linking)
VI  - 28
IP  - 9
DP  - 2014 May
TI  - [Expression and significance of the cell cycle regulators in laryngeal 
      carcinogenesis detected by flow cytometry].
PG  - 635-7
AB  - OBJECTIVE: To investigate the expression and clinicopathological significance of 
      the cell cycle regulators cyclin E, cyclin D1, p21, p16 in laryngeal 
      carcinogenesis tissus. METHOD: The expression of cell cycle regulators were 
      detected by flow cytometry method in 23 cases of polyps of vocal cord, 69 cases 
      of laryngeal precancerous change and 33 cases of laryngeal squamous cell 
      carcinoma (LSCC), which tissue was paraffin embedded, sliced, dewaxed, and 
      prepared into the cell suspension, then fluorescently labeled by cyclin E, cyclin 
      D1, p21 and p16. RESULT: In polyps of vocal cord, laryngeal precancerous change 
      and LSCC, The positive expression rate of cyclin E and cyclin D1 were 
      respectively 13.04%, 20.29D, 42.420 and 26.09%, 43.48% and 93.94%. The positive 
      expression rate of p16 and p21 were respectively 61.90%, 40.98%, 14.28% and 
      47.62%, 23.81%, 26.23%. Those showed the positive expression rate of cyclin D1, 
      cyclin E gradually decreased from vocal cord polyps, laryngeal precancerous 
      change to LSCC, (P < 0.05, P < 0.01), while the positive expression rate of p21 
      and p16 gradually decreased (P < 0.01). CONCLUSION: The abnormal expression of 
      cell cycle regulatory factors is the molecular events of laryngeal carcinoma. 
      High expression of positive regulatory factors cyclin D1 and cyclin E, and low 
      expression of negative regulatory factors p16 and p21, which showed the imbalance 
      of multiple positive and negative regulatory factors related with cell cycle play 
      an important role in the occurrence of laryngeal cancer.
FAU - Chen, Caifeng
AU  - Chen C
FAU - Li, Yunying
AU  - Li Y
FAU - Huang, Yu
AU  - Huang Y
FAU - He, Qinglian
AU  - He Q
FAU - Lin, Wenmin
AU  - Lin W
FAU - Lu, Biaoqing
AU  - Lu B
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical 
      otorhinolaryngology, head, and neck surgery
JID - 101303164
RN  - 0 (CCND1 protein, human)
RN  - 0 (CCNE1 protein, human)
RN  - 0 (Cyclin E)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Oncogene Proteins)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adult
MH  - Cyclin D1/*metabolism
MH  - Cyclin E/*metabolism
MH  - Cyclin-Dependent Kinase Inhibitor p16/*metabolism
MH  - Cyclin-Dependent Kinase Inhibitor p21/*metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Laryngeal Neoplasms/metabolism/*pathology
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins/*metabolism
EDAT- 2014/09/10 06:00
MHDA- 2015/01/15 06:00
CRDT- 2014/09/09 06:00
PHST- 2014/09/09 06:00 [entrez]
PHST- 2014/09/10 06:00 [pubmed]
PHST- 2015/01/15 06:00 [medline]
PST - ppublish
SO  - Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2014 May;28(9):635-7.