PMID- 25196350
OWN - NLM
STAT- MEDLINE
DCOM- 20160412
LR  - 20211021
IS  - 1479-5876 (Electronic)
IS  - 1479-5876 (Linking)
VI  - 12
DP  - 2014 Sep 8
TI  - Multiple injections of human umbilical cord-derived mesenchymal stromal cells 
      through the tail vein improve microcirculation and the microenvironment in a rat 
      model of radiation myelopathy.
PG  - 246
LID - 10.1186/s12967-014-0246-6 [doi]
LID - 246
AB  - BACKGROUND: At present, no effective clinical treatment is available for the late 
      effects of radiation myelopathy. The aim of the present study was to assess the 
      therapeutic effects of human umbilical cord-derived mesenchymal stromal cells 
      (UC-MSCs) in a rat model of radiation myelopathy. METHODS: An irradiated cervical 
      spinal cord rat model was generated. UC-MSCs were injected through the tail vein 
      at 90, 97, 104 and 111 days post-irradiation. Behavioral tests were performed 
      using the forelimb paralysis scoring system, and histological damage was examined 
      using Nissl staining. The microcirculation in the spinal cord was assessed using 
      von Willebrand factor (vWF) immunohistochemical analysis and laser-Doppler 
      flowmetry. The microenvironment in the spinal cord was determined by measuring 
      the pro-inflammatory cytokines interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha 
      (TNF-alpha) in the serum and the anti-inflammatory cytokines brain-derived 
      neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) in 
      the spinal cord. RESULTS: Multiple injections of UC-MSCs through the tail veil 
      decreased the forelimb paralysis, decreased spinal cord histological damage, 
      increased the number of neurons in the anterior horn of the spinal cord, 
      increased the endothelial cell density and the microvessel density in the white 
      matter and gray matter of the spinal cord, increased the relative magnitude of 
      spinal cord blood flow, down-regulated pro-inflammatory cytokine expression in 
      the serum, and increased anti-inflammatory cytokine expression in the spinal 
      cord. CONCLUSION: Multiple injections of UC-MSCs via the tail vein in a rat model 
      of radiation myelopathy significantly improved the microcirculation and 
      microenvironment through therapeutic paracrine effects.
FAU - Wei, Li
AU  - Wei L
FAU - Zhang, Jing
AU  - Zhang J
FAU - Xiao, Xiu-Bin
AU  - Xiao XB
FAU - Mai, Hai-Xing
AU  - Mai HX
FAU - Zheng, Ke
AU  - Zheng K
FAU - Sun, Wan-Liang
AU  - Sun WL
FAU - Wang, Lei
AU  - Wang L
FAU - Liang, Feng
AU  - Liang F
FAU - Yang, Zai-Liang
AU  - Yang ZL
FAU - Liu, Yuan
AU  - Liu Y
FAU - Wang, Yan-Qing
AU  - Wang YQ
FAU - Li, Zhi-Fang
AU  - Li ZF
FAU - Wang, Jia-Ning
AU  - Wang JN
FAU - Zhang, Wei-Jing
AU  - Zhang WJ
FAU - You, Hua
AU  - You H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140908
PL  - England
TA  - J Transl Med
JT  - Journal of translational medicine
JID - 101190741
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Disease Models, Animal
MH  - *Mesenchymal Stem Cell Transplantation
MH  - *Mesenchymal Stem Cells
MH  - *Microcirculation
MH  - Radiation Injuries, Experimental/*therapy
MH  - Rats
MH  - Spinal Cord/physiopathology
MH  - Spinal Cord Diseases/*therapy
MH  - Tail/*blood supply
MH  - Umbilical Cord/*cytology
MH  - Veins
PMC - PMC4174271
EDAT- 2014/09/10 06:00
MHDA- 2016/04/14 06:00
PMCR- 2014/09/08
CRDT- 2014/09/09 06:00
PHST- 2014/07/05 00:00 [received]
PHST- 2014/08/28 00:00 [accepted]
PHST- 2014/09/09 06:00 [entrez]
PHST- 2014/09/10 06:00 [pubmed]
PHST- 2016/04/14 06:00 [medline]
PHST- 2014/09/08 00:00 [pmc-release]
AID - s12967-014-0246-6 [pii]
AID - 246 [pii]
AID - 10.1186/s12967-014-0246-6 [doi]
PST - epublish
SO  - J Transl Med. 2014 Sep 8;12:246. doi: 10.1186/s12967-014-0246-6.