PMID- 25197330 OWN - NLM STAT- MEDLINE DCOM- 20150609 LR - 20211021 IS - 1936-2625 (Electronic) IS - 1936-2625 (Linking) VI - 7 IP - 8 DP - 2014 TI - Venlafaxine inhibits apoptosis of hippocampal neurons by up-regulating brain-derived neurotrophic factor in a rat depression model. PG - 4577-86 AB - OBJECTIVE: To study the effect of venlafaxine on the expression of brain-derived neurotrophic factor (BDNF) in rat hippocampal neurons, as well as its inhibitory effect on apoptosis of hippocampal neurons. METHODS: Differences in behavioral ability between the depression model group and the Venlafaxine treatment group were observed using behavioral, sucrose-water and open field tests. The rat hippocampal tissue was sliced, stained and observed for BDNF distribution by immunohistochemistry. Apoptosis of hippocampal neurons was detected by TUNEL. BDNF expression in the hippocampal tissue was detected by Western blot. Injury and apoptosis of the hippocampal tissue were observed by electron microscopy. RESULTS: Behavioral test showed that venlafaxine effectively improved the behavioral abilities of depressed rats. Immunohistochemistry showed that venlafaxine markedly increased the BDNF expression in the rat hippocampus. TUNEL showed that venlafaxine markedly inhibited apoptosis of hippocampal neurons, which was also confirmed by electron microscopic observation of the pathologic sections. CONCLUSION: Venlafaxine improved the expression of BDNF through working on PI3k/PKB/eNOS pathway and repressed the apoptosis of hippocampal neurons. FAU - Huang, Xiao AU - Huang X AD - Department of Psychological Medicine, Zhongshan Hospital, Fudan University Shanghai, China. FAU - Mao, Yue-Shi AU - Mao YS AD - Department of Neurology, Zhongshan Hospital, Fudan University Shanghai, China. FAU - Li, Chao AU - Li C AD - Shanghai Mental Health Center, Shanghai Jiaotong University Shanghai, China. FAU - Wang, Hao AU - Wang H AD - The Experimental Center of Basic Medicine, Shanghai Medical College, Fudan University Shanghai 200032, China. FAU - Ji, Jian-Lin AU - Ji JL AD - Department of Psychological Medicine, Zhongshan Hospital, Fudan University Shanghai, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140715 PL - United States TA - Int J Clin Exp Pathol JT - International journal of clinical and experimental pathology JID - 101480565 RN - 0 (Antidepressive Agents, Second-Generation) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Cyclohexanols) RN - 7D7RX5A8MO (Venlafaxine Hydrochloride) SB - IM MH - Animals MH - Antidepressive Agents, Second-Generation/*pharmacology MH - Apoptosis/*drug effects MH - Behavior, Animal/drug effects MH - Blotting, Western MH - Brain-Derived Neurotrophic Factor/*biosynthesis MH - Cyclohexanols/*pharmacology MH - Depression/metabolism/*pathology MH - Disease Models, Animal MH - Hippocampus/*drug effects/pathology MH - Immunohistochemistry MH - In Situ Nick-End Labeling MH - Male MH - Microscopy, Electron, Transmission MH - Neurons/*drug effects/pathology MH - Rats MH - Rats, Sprague-Dawley MH - Up-Regulation MH - Venlafaxine Hydrochloride PMC - PMC4152020 OTO - NOTNLM OT - Venlafaxine OT - apoptosis OT - brain-derived neurotrophic factor (BDNF) OT - hippocampal neuron EDAT- 2014/09/10 06:00 MHDA- 2015/06/10 06:00 PMCR- 2014/07/15 CRDT- 2014/09/09 06:00 PHST- 2014/05/16 00:00 [received] PHST- 2014/07/12 00:00 [accepted] PHST- 2014/09/09 06:00 [entrez] PHST- 2014/09/10 06:00 [pubmed] PHST- 2015/06/10 06:00 [medline] PHST- 2014/07/15 00:00 [pmc-release] PST - epublish SO - Int J Clin Exp Pathol. 2014 Jul 15;7(8):4577-86. eCollection 2014.