PMID- 25198921
OWN - NLM
STAT- MEDLINE
DCOM- 20160310
LR  - 20231213
IS  - 1520-5126 (Electronic)
IS  - 0002-7863 (Linking)
VI  - 136
IP  - 42
DP  - 2014 Oct 22
TI  - A gold nanoparticle pentapeptide: gene fusion to induce therapeutic gene 
      expression in mesenchymal stem cells.
PG  - 14763-71
LID - 10.1021/ja505190q [doi]
AB  - Mesenchymal stem cells (MSC) have been identified as having great potential as 
      autologous cell therapeutics to treat traumatic brain injury and spinal injury as 
      well as neuronal and cardiac ischemic events. All future clinical applications of 
      MSC cell therapies must allow the MSC to be harvested, transfected, and induced 
      to express a desired protein or selection of proteins to have medical benefit. 
      For the full potential of MSC cell therapy to be realized, it is desirable to 
      systematically alter the protein expression of therapeutically beneficial 
      biomolecules in harvested MSC cells with high fidelity in a single transfection 
      event. We have developed a delivery platform on the basis of the use of a solid 
      gold nanoparticle that has been surface modified to produce a fusion containing a 
      zwitterionic, pentapeptide designed from Bax inhibiting peptide (Ku70) to enhance 
      cellular uptake and a linearized expression vector to induce enhanced expression 
      of brain-derived neurotrophic factor (BDNF) in rat-derived MSCs. Ku70 is observed 
      to effect >80% transfection following a single treatment of femur bone marrow 
      isolated rat MSCs with efficiencies for the delivery of a 6.6 kbp gene on either 
      a Au nanoparticle (NP) or CdSe/ZnS quantum dot (QD). Gene expression is observed 
      within 4 d by optical measurements, and secretion is observed within 10 d by 
      Western Blot analysis. The combination of being able to selectively engineer the 
      NP, to colocalize biological agents, and to enhance the stability of those agents 
      has provided the strong impetus to utilize this novel class of materials to 
      engineer primary MSCs.
FAU - Muroski, Megan E
AU  - Muroski ME
AD  - Department of Chemistry and Biochemistry, 95 Chieftan Way, Florida State 
      University , Tallahassee, Florida 32306-4390, United States.
FAU - Morgan, Thomas J Jr
AU  - Morgan TJ Jr
FAU - Levenson, Cathy W
AU  - Levenson CW
FAU - Strouse, Geoffrey F
AU  - Strouse GF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20141007
PL  - United States
TA  - J Am Chem Soc
JT  - Journal of the American Chemical Society
JID - 7503056
RN  - 0 (Antigens, Nuclear)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drug Carriers)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Oligopeptides)
RN  - 0 (Peptide Fragments)
RN  - 7440-57-5 (Gold)
RN  - EC 3.6.4.12 (Xrcc6 protein, rat)
RN  - EC 4.2.99.- (Ku Autoantigen)
SB  - IM
MH  - Animals
MH  - Antigens, Nuclear/chemistry
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - Cell- and Tissue-Based Therapy
MH  - DNA-Binding Proteins/chemistry
MH  - Drug Carriers/*chemistry
MH  - Gene Expression
MH  - *Gene Fusion
MH  - Gold/*chemistry
MH  - Ku Autoantigen
MH  - Luminescent Proteins/genetics
MH  - Mesenchymal Stem Cells/*metabolism
MH  - *Metal Nanoparticles
MH  - Oligopeptides/*chemistry
MH  - Peptide Fragments/chemistry
MH  - Quantum Dots/chemistry
MH  - Rats
MH  - Transfection/*methods
MH  - Red Fluorescent Protein
EDAT- 2014/09/10 06:00
MHDA- 2016/03/11 06:00
CRDT- 2014/09/09 06:00
PHST- 2014/09/09 06:00 [entrez]
PHST- 2014/09/10 06:00 [pubmed]
PHST- 2016/03/11 06:00 [medline]
AID - 10.1021/ja505190q [doi]
PST - ppublish
SO  - J Am Chem Soc. 2014 Oct 22;136(42):14763-71. doi: 10.1021/ja505190q. Epub 2014 
      Oct 7.