PMID- 25200330
OWN - NLM
STAT- MEDLINE
DCOM- 20150622
LR  - 20220331
IS  - 1558-9307 (Electronic)
IS  - 0024-4201 (Linking)
VI  - 49
IP  - 10
DP  - 2014 Oct
TI  - High dietary cholesterol masks type 2 diabetes-induced osteopenia and changes in 
      bone microstructure in rats.
PG  - 975-86
LID - 10.1007/s11745-014-3950-3 [doi]
AB  - Type 2 diabetes mellitus (T2DM) often occurs concurrently with high blood 
      cholesterol or dyslipidemia. Although T2DM has been hypothesized to impair bone 
      microstructure, several investigations showed that, when compared to age-matched 
      healthy individuals, T2DM patients had normal or relatively high bone mineral 
      density (BMD). Since cholesterol and lipids profoundly affect the function of 
      osteoblasts and osteoclasts, it might be cholesterol that obscured the changes in 
      BMD and bone microstructure in T2DM. The present study, therefore, aimed to 
      determine bone elongation, epiphyseal histology, and bone microstructure in 
      non-obese T2DM Goto-Kakizaki rats treated with normal (GK-ND) and high 
      cholesterol diet. We found that volumetric BMD was lower in GK-ND rats than the 
      age-matched wild-type controls. In histomorphometric study of tibial metaphysis, 
      T2DM evidently suppressed osteoblast function as indicated by decreases in 
      osteoblast surface, mineral apposition rate, and bone formation rate in GK-ND 
      rats. Meanwhile, the osteoclast surface and eroded surface were increased in 
      GK-ND rats, thus suggesting an activation of bone resorption. T2DM also impaired 
      bone elongation, presumably by retaining the chondrogenic precursor cells in the 
      epiphyseal resting zone. Interestingly, several bone changes in GK rats (e.g., 
      increased osteoclast surface) disappeared after high cholesterol treatment as 
      compared to wild-type rats fed high cholesterol diet. In conclusion, high 
      cholesterol diet was capable of masking the T2DM-induced osteopenia and changes 
      in several histomorphometric parameters that indicated bone microstructural 
      defect. Cholesterol thus explained, in part, why a decrease in BMD was not 
      observed in T2DM, and hence delayed diagnosis of the T2DM-associated bone 
      disease.
FAU - Lapmanee, Sarawut
AU  - Lapmanee S
AD  - Department of Physiology, Faculty of Science, Mahidol University, Rama VI Road, 
      Bangkok, 10400, Thailand.
FAU - Charoenphandhu, Narattaphol
AU  - Charoenphandhu N
FAU - Aeimlapa, Ratchaneevan
AU  - Aeimlapa R
FAU - Suntornsaratoon, Panan
AU  - Suntornsaratoon P
FAU - Wongdee, Kannikar
AU  - Wongdee K
FAU - Tiyasatkulkovit, Wacharaporn
AU  - Tiyasatkulkovit W
FAU - Kengkoom, Kanchana
AU  - Kengkoom K
FAU - Chaimongkolnukul, Khuanjit
AU  - Chaimongkolnukul K
FAU - Seriwatanachai, Dutmanee
AU  - Seriwatanachai D
FAU - Krishnamra, Nateetip
AU  - Krishnamra N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140909
PL  - United States
TA  - Lipids
JT  - Lipids
JID - 0060450
RN  - 0 (Cholesterol, Dietary)
SB  - IM
MH  - Animals
MH  - Bone Density
MH  - Bone Diseases, Metabolic/diagnosis/*etiology/metabolism
MH  - Bone and Bones/diagnostic imaging/pathology
MH  - Cholesterol, Dietary/*administration & dosage
MH  - Delayed Diagnosis
MH  - Diabetes Mellitus, Type 2/*complications/metabolism
MH  - Dyslipidemias/complications/metabolism
MH  - Female
MH  - Growth Plate/pathology
MH  - Lipid Metabolism
MH  - Osteoblasts/pathology
MH  - Osteoclasts/pathology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - X-Ray Microtomography
EDAT- 2014/09/10 06:00
MHDA- 2015/06/24 06:00
CRDT- 2014/09/10 06:00
PHST- 2014/03/25 00:00 [received]
PHST- 2014/08/27 00:00 [accepted]
PHST- 2014/09/10 06:00 [entrez]
PHST- 2014/09/10 06:00 [pubmed]
PHST- 2015/06/24 06:00 [medline]
AID - 10.1007/s11745-014-3950-3 [doi]
PST - ppublish
SO  - Lipids. 2014 Oct;49(10):975-86. doi: 10.1007/s11745-014-3950-3. Epub 2014 Sep 9.