PMID- 25201136
OWN - NLM
STAT- MEDLINE
DCOM- 20150702
LR  - 20220409
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 45
IP  - 6
DP  - 2014 Dec
TI  - HP1beta suppresses metastasis of human cancer cells by decreasing the expression and 
      activation of MMP2.
PG  - 2541-8
LID - 10.3892/ijo.2014.2646 [doi]
AB  - Heterochromatin protein 1 (HP1) is an epigenetic modifier of gene regulation and 
      chromatin packing via binding to trimethylated histone H3 lysine 9 (H3K9). HP1 
      plays an important role in gene activation as well as gene repression in 
      heterochromatin and euchromatin. However, the role of individual HP1 proteins in 
      human diseases remains elusive. Here, we show that HP1beta negatively regulates the 
      expression and activation of matrix metallopeptidase (MMP)2, which mediates 
      cancer metastasis by destructing type Ⅳ collagen. Reduced HP1beta expression 
      correlates with the increased level of pro- and active-MMP2 in colon cancer 
      cells. Consistently, HP1beta knockdown (KD) increased and HP1beta overexpression 
      decreased the mRNA level of MMP2 and membrane type 1 metallopeptidase (MT1-MMP). 
      Furthermore, cancer cells overexpressing HP1beta showed impaired migratory ability, 
      whereas HP1beta‑deleted cancer cells had increased migration. HP1beta negatively 
      regulates MMP2 expression in a transcriptional level and prevents MMP2 activation 
      through reducing the expression of MT1‑MMP. These findings shed new light on HP1beta 
      as a molecular regulator and an efficient therapeutic target of metastatic 
      cancer.
FAU - Yi, Sang Ah
AU  - Yi SA
AD  - Research Center for Epigenome Regulation, School of Pharmacy, Sungkyunkwan 
      University, Suwon, Gyeonggi 440‑746, Republic of Korea.
FAU - Ryu, Hyun-Wook
AU  - Ryu HW
AD  - College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei 
      University, Incheon 406‑840, Republic of Korea.
FAU - Lee, Dong Hoon
AU  - Lee DH
AD  - College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei 
      University, Incheon 406‑840, Republic of Korea.
FAU - Han, Jeung-Whan
AU  - Han JW
AD  - Research Center for Epigenome Regulation, School of Pharmacy, Sungkyunkwan 
      University, Suwon, Gyeonggi 440‑746, Republic of Korea.
FAU - Kwon, So Hee
AU  - Kwon SH
AD  - College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei 
      University, Incheon 406‑840, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140909
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (CBX1 protein, human)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Histones)
RN  - 107283-02-3 (Chromobox Protein Homolog 5)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.80 (MMP14 protein, human)
RN  - EC 3.4.24.80 (Matrix Metalloproteinase 14)
SB  - IM
MH  - Cell Movement/*genetics
MH  - Chromobox Protein Homolog 5
MH  - Chromosomal Proteins, Non-Histone/biosynthesis/*genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Histones/genetics
MH  - Humans
MH  - MCF-7 Cells
MH  - Matrix Metalloproteinase 14/biosynthesis
MH  - Matrix Metalloproteinase 2/*biosynthesis
MH  - Neoplasm Invasiveness/*genetics
MH  - Neoplasm Metastasis
MH  - Neoplasms
MH  - Phosphatidylinositol 3-Kinases/biosynthesis
MH  - Signal Transduction
EDAT- 2014/09/10 06:00
MHDA- 2015/07/03 06:00
CRDT- 2014/09/10 06:00
PHST- 2014/07/07 00:00 [received]
PHST- 2014/08/26 00:00 [accepted]
PHST- 2014/09/10 06:00 [entrez]
PHST- 2014/09/10 06:00 [pubmed]
PHST- 2015/07/03 06:00 [medline]
AID - 10.3892/ijo.2014.2646 [doi]
PST - ppublish
SO  - Int J Oncol. 2014 Dec;45(6):2541-8. doi: 10.3892/ijo.2014.2646. Epub 2014 Sep 9.