PMID- 25201703
OWN - NLM
STAT- MEDLINE
DCOM- 20150803
LR  - 20171116
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 59
IP  - 12
DP  - 2014 Dec
TI  - The impact of nitric oxide on calcium homeostasis in PE/CA-PJ15 cells.
PG  - 1377-83
LID - S0003-9969(14)00207-6 [pii]
LID - 10.1016/j.archoralbio.2014.07.022 [doi]
AB  - OBJECTIVE: Nitric oxide (NO) production and Ca(2+) homeostasis are key 
      determinants for the control of many cell functions. NO is known to be a mediator 
      of Ca(2+) homeostasis in a highly complex and cell-specific manner and although 
      Ca(2+) homeostasis has been explored in human oral cancer cells, the exact 
      mechanisms are not completely understood. In this study we investigated the 
      impact of exogenous NO on [Ca(2+)]c homeostasis in PE/CA-PJ15 cells. DESIGN: 
      Cells were treated with S-nitrosocysteine as NO-donor and the determinations of 
      cytosolic Ca(2+) concentrations were performed using FURA-2 AM. Carbonyl cyanide 
      p-(trifluoromethoxy) phenylhydrazone (FCCP) and oligomycin were used to challenge 
      mitochondrial functionality, whereas thapsigargin (TG) and La(3+) were employed 
      to perturb intracellular calcium levels. RESULTS: NO derived from 
      S-nitrosocysteine (CySNO) induced a dose-dependent reduction of cytosolic calcium 
      [Ca(2+)]c whereas oxy-haemoglobin (oxyHb) completely counteracted this effect. 
      Subsequently, we assessed possible relationships between NO and cellular 
      structures responsible for Ca(2+) homeostasis. We found that uncoupling of 
      mitochondrial respiration with 
      carbonyl-cyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) and oligomycin 
      strongly reduced the effect of NO on [Ca(2+)]c. Moreover, we found that during 
      this mitochondrial energetic deficit, the effect of NO on [Ca(2+)]c was also 
      reduced in the presence of La(3+) or thapsigargin. CONCLUSIONS: NO induces a 
      concentration-dependent [Ca(2+)]c reduction in PE/CA-PJ15 human oral cancer cells 
      and potentiates mitochondrial Ca(2+) buffering in the presence of TG or La(3+). 
      Further, we show that exogenous NO deregulates Ca(2+) homeostasis in PE/CA-PJ15 
      cells with fully energized mitochondria.
CI  - Copyright (c) 2014. Published by Elsevier Ltd.
FAU - Tiribuzi, Roberto
AU  - Tiribuzi R
AD  - Dipartimento di Medicina Sperimentale e Scienze Biochimiche, University of 
      Perugia, Italy. Electronic address: carlo.palmerini@unipg.it.
FAU - Tartacca, Francesco
AU  - Tartacca F
AD  - Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Research Unit of 
      Biochemistry and Molecular Biology, University of Perugia, Perugia, Italy.
FAU - Aisa, Maria Cristina
AU  - Aisa MC
AD  - Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Research Unit of 
      Biochemistry and Molecular Biology, University of Perugia, Perugia, Italy.
FAU - Cerulli, Giuliano Giorgio
AU  - Cerulli GG
AD  - Dipartimento di Specialita Medico Chirurgiche e Sanita pubblica, University of 
      Perugia, Perugia, Italy.
FAU - Palmerini, Carlo Alberto
AU  - Palmerini CA
AD  - Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Research Unit of 
      Biochemistry and Molecular Biology, University of Perugia, Perugia, Italy.
LA  - eng
PT  - Journal Article
DEP - 20140807
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Oligomycins)
RN  - 0 (Oxyhemoglobins)
RN  - 0 (S-Nitrosothiols)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 370-86-5 (Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone)
RN  - 67526-95-8 (Thapsigargin)
RN  - 926P2322P4 (S-nitrosocysteine)
RN  - K848JZ4886 (Cysteine)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Calcium Signaling/*drug effects
MH  - Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone/pharmacology
MH  - Cell Survival/drug effects
MH  - Cysteine/analogs & derivatives/pharmacology
MH  - Cytosol/chemistry
MH  - Dose-Response Relationship, Drug
MH  - Fura-2/pharmacology
MH  - Homeostasis/drug effects
MH  - Humans
MH  - Mitochondria
MH  - Nitric Oxide/*pharmacology
MH  - Oligomycins/pharmacology
MH  - Oxyhemoglobins/pharmacology
MH  - S-Nitrosothiols/pharmacology
MH  - Thapsigargin/pharmacology
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - Cytosolic calcium
OT  - Endoplasmic reticulum
OT  - Mitochondrion
OT  - Nitric oxide
EDAT- 2014/09/10 06:00
MHDA- 2015/08/04 06:00
CRDT- 2014/09/10 06:00
PHST- 2014/02/06 00:00 [received]
PHST- 2014/07/29 00:00 [revised]
PHST- 2014/07/30 00:00 [accepted]
PHST- 2014/09/10 06:00 [entrez]
PHST- 2014/09/10 06:00 [pubmed]
PHST- 2015/08/04 06:00 [medline]
AID - S0003-9969(14)00207-6 [pii]
AID - 10.1016/j.archoralbio.2014.07.022 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2014 Dec;59(12):1377-83. doi: 10.1016/j.archoralbio.2014.07.022. 
      Epub 2014 Aug 7.