PMID- 25202830 OWN - NLM STAT- MEDLINE DCOM- 20150731 LR - 20181202 IS - 1873-3360 (Electronic) IS - 0306-4530 (Linking) VI - 50 DP - 2014 Dec TI - The beneficial effects of strength exercise on hippocampal cell proliferation and apoptotic signaling is impaired by anabolic androgenic steroids. PG - 106-17 LID - S0306-4530(14)00315-1 [pii] LID - 10.1016/j.psyneuen.2014.08.009 [doi] AB - Previous studies have shown that strength exercise improves memory and increases expression of a myriad of proteins involved on neuronal survival and synaptic plasticity in the hippocampus. Conversely, chronic exposure to supraphysiological levels of anabolic androgenic steroids (AAS) can induce psychiatric abnormalities, cognitive deficits, impair neurotransmission, alter the levels of neurotrophic factors, decrease cell proliferation and neurogenesis, and enhance neuronal cell death. In the present study, we investigated the effects of the AAS nandrolone decanoate (ND) administration during a strength exercise program on cell proliferation, apoptotic status and brain-derived neurotrophic factor (BDNF) expression in the rat hippocampus. Adult male Wistar rats were subjected to 4 weeks of progressive strength exercise in a vertical ladder apparatus with or without daily doses (5.0 mg/kg, SC) of ND. Immunohistochemistry analysis revealed that strength exercise increased significantly the number of Ki-67-positive cells (a cell proliferation marker) in dentate gyrus (DG) of hippocampus. However, this effect was abrogated when strength exercise was combined with ND. Although western blot analysis of whole hippocampus showed no significant differences in Bax and Bcl-2 protein expression among groups, the immunoreactivity of the pro-apoptotic protein Bax was significantly increased in DG, CA1 and CA3 hippocampal subfields of sedentary rats treated with ND. Moreover, the increase in the immunoreactivity of anti-apoptotic protein Bcl-2 (DG and CA3) induced by strength exercise was diminished by ND. There were no significant differences in BDNF expression among experimental groups. Therefore, the present findings suggest that the beneficial effects of strength exercise on hippocampal cell proliferation and apoptotic signaling are impaired by ND. CI - Copyright (c) 2014 Elsevier Ltd. All rights reserved. FAU - Novaes Gomes, Fabiano Guimaraes AU - Novaes Gomes FG AD - Department of Physiology, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, SP 04023-900, Brazil; Department of Exercise Physiology, Faculdade de Minas (FAMINAS), Muriae-Minas Gerais, 36880-000, Brazil. FAU - Fernandes, Jansen AU - Fernandes J AD - Department of Physiology, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, SP 04023-900, Brazil. FAU - Vannucci Campos, Diego AU - Vannucci Campos D AD - Department of Physiology, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, SP 04023-900, Brazil. FAU - Cassilhas, Ricardo Cardoso AU - Cassilhas RC AD - Department of Psychobiology, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo 04023-900, Brazil. FAU - Viana, Gustavo Monteiro AU - Viana GM AD - Department of Psychobiology, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo 04023-900, Brazil. FAU - D'Almeida, Vania AU - D'Almeida V AD - Department of Psychobiology, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo 04023-900, Brazil. FAU - de Moraes Rego, Marta Karavisch AU - de Moraes Rego MK AD - Department of Physiology, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, SP 04023-900, Brazil. FAU - Buainain, Pedro Ivo AU - Buainain PI AD - Department of Physiology, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, SP 04023-900, Brazil. FAU - Cavalheiro, Esper Abrao AU - Cavalheiro EA AD - Department of Neurology and Neurosurgery, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo 04023-900, Brazil. FAU - Arida, Ricardo Mario AU - Arida RM AD - Department of Physiology, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, SP 04023-900, Brazil. Electronic address: arida.nexp@epm.br. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140822 PL - England TA - Psychoneuroendocrinology JT - Psychoneuroendocrinology JID - 7612148 RN - 0 (Anabolic Agents) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Proto-Oncogene Proteins c-bcl-2) RN - 0 (bcl-2-Associated X Protein) RN - 6PG9VR430D (Nandrolone) RN - H45187T098 (Nandrolone Decanoate) SB - IM MH - Anabolic Agents/*pharmacology MH - Animals MH - Apoptosis/*drug effects MH - Brain-Derived Neurotrophic Factor/metabolism MH - Cell Proliferation/*drug effects/physiology MH - Hippocampus/cytology/*drug effects/metabolism MH - Male MH - Nandrolone/*analogs & derivatives/pharmacology MH - Nandrolone Decanoate MH - Physical Conditioning, Animal/*physiology MH - Proto-Oncogene Proteins c-bcl-2/metabolism MH - Rats MH - Rats, Wistar MH - *Resistance Training MH - bcl-2-Associated X Protein/metabolism OTO - NOTNLM OT - Anabolic androgenic steroids OT - Apoptosis OT - BDNF OT - Hippocampus OT - Neuroplasticity OT - Strength exercise EDAT- 2014/09/10 06:00 MHDA- 2015/08/01 06:00 CRDT- 2014/09/10 06:00 PHST- 2014/01/30 00:00 [received] PHST- 2014/08/09 00:00 [revised] PHST- 2014/08/09 00:00 [accepted] PHST- 2014/09/10 06:00 [entrez] PHST- 2014/09/10 06:00 [pubmed] PHST- 2015/08/01 06:00 [medline] AID - S0306-4530(14)00315-1 [pii] AID - 10.1016/j.psyneuen.2014.08.009 [doi] PST - ppublish SO - Psychoneuroendocrinology. 2014 Dec;50:106-17. doi: 10.1016/j.psyneuen.2014.08.009. Epub 2014 Aug 22.