PMID- 25202882 OWN - NLM STAT- MEDLINE DCOM- 20151215 LR - 20150330 IS - 1552-4965 (Electronic) IS - 1549-3296 (Linking) VI - 103 IP - 5 DP - 2015 May TI - Use of polycaprolactone (PCL) as scaffolds for the regeneration of nerve tissue. PG - 1755-60 LID - 10.1002/jbm.a.35318 [doi] AB - Adipose tissue is an easily accessible source of stem cells for use in tissue regenerative medicine. In the literature, different methods have been used to stimulate acquisition of neuronal characteristics by adipose-derived stem cells (ADSC). Herein we study the growth and neuronal differentiation potential of ADSC seeded onto a porous polycaprolactone (PCL) scaffold. The objective of this study is to demonstrate that PCL can be used as a scaffold to support reconstruction of new nervous tissue using adipose stem cells. We have previously shown that undifferentiated ADSC adhere and grow on PCL. Herein we show that, after culture on PCL in neuronal differentiation medium, ADSC expressed molecular markers characteristic of neuronal cells (beta-tubulin-III, Neuron-Specific Enolase (NSE), Nestin) and secrete brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF). This study suggests that PCL can be used as a scaffold to generate nervous tissue in vitro. PLC has excellent mechanical properties and a slow degradation rate. Moreover, on the basis of our results, we propose that PCL could be used for to make in vitro, scaffold coated with neuronal cells derived from Adipose stem cells (ADSC). Neuronal cells-coated PCL could find several applications to replace damaged area of ​​the body; for example, a possible use could be the generation of nerves. CI - (c) 2014 Wiley Periodicals, Inc. FAU - Barbarisi, Manlio AU - Barbarisi M AD - Laboratory of Applied Biotechnology, Department of Anaesthesiological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy. FAU - Marino, Gerardo AU - Marino G FAU - Armenia, Emilia AU - Armenia E FAU - Vincenzo, Quagliariello AU - Vincenzo Q FAU - Rosso, Francesco AU - Rosso F FAU - Porcelli, Marina AU - Porcelli M FAU - Barbarisi, Alfonso AU - Barbarisi A LA - eng PT - Journal Article DEP - 20140915 PL - United States TA - J Biomed Mater Res A JT - Journal of biomedical materials research. Part A JID - 101234237 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Polyesters) RN - 24980-41-4 (polycaprolactone) RN - 9061-61-4 (Nerve Growth Factor) SB - IM MH - Adipose Tissue/cytology MH - Adult MH - Brain-Derived Neurotrophic Factor/metabolism MH - Cell Proliferation/drug effects MH - Flow Cytometry MH - Humans MH - Male MH - Microscopy, Fluorescence MH - Middle Aged MH - Nerve Growth Factor/metabolism MH - Nerve Regeneration/*drug effects MH - Nerve Tissue/drug effects/*physiology MH - Neurons/cytology/drug effects/metabolism MH - Polyesters/*pharmacology MH - Stem Cells/cytology/drug effects/ultrastructure MH - Tissue Scaffolds/*chemistry OTO - NOTNLM OT - adipose stem cells OT - nerve OT - regeneration OT - stem cells EDAT- 2014/09/10 06:00 MHDA- 2015/12/17 06:00 CRDT- 2014/09/10 06:00 PHST- 2014/05/07 00:00 [received] PHST- 2014/08/01 00:00 [revised] PHST- 2014/08/26 00:00 [accepted] PHST- 2014/09/10 06:00 [entrez] PHST- 2014/09/10 06:00 [pubmed] PHST- 2015/12/17 06:00 [medline] AID - 10.1002/jbm.a.35318 [doi] PST - ppublish SO - J Biomed Mater Res A. 2015 May;103(5):1755-60. doi: 10.1002/jbm.a.35318. Epub 2014 Sep 15.