PMID- 25202945
OWN - NLM
STAT- MEDLINE
DCOM- 20150908
LR  - 20181202
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 39
IP  - 8
DP  - 2014 Aug
TI  - [Effect of Tip30 expression on the invasion and metastasis of hepatoma cells].
PG  - 775-83
LID - 10.3969/j.issn.1672-7347.2014.08.004 [doi]
AB  - OBJECTIVE: To investigate the effect of Tip30 on the invasion and metastasis of 
      hepatoma cells. METHODS: Recombinant plasmid pAAV-Tip30 was transfected to HepG2 
      cells by polylactic-coglycolic acid (PLGA) nanoparticles. RT-PCR was used to 
      detect the mRNA expression of matrix metalloproteinases (MMP)-2, MMP-9 and 
      epidermal growth factor receptor (EGFR). MMP- 2 and MMP-9 protein level in the 
      tumor tissue was detected by Western blot. The proliferation, of hepatoma cells 
      was evaluated by MTS assay and clone formation method. The adhesion and invasion 
      of hepatoma cells were evaluated by adhesion assay and Transwell migration assay, 
      respectively. RESULTS: With RT-PCR and Western blot, we found Tip30 decreased the 
      expression of MMP-2 and MMP-9. MTS assay and cell clone formation method showed 
      inhibited proliferation of the HepG2 cells. Chemo invasion assays showed that 
      Tip30 decreased the invasiveness of hepatoma cells. Tip30 attenuated the binding 
      of liver cancer cell line to human umbilical vein endothelial cells and 
      fibronectin. CONCLUSION: The invasion and metastasis of hepatoma cells can be 
      inhibited by Tip30 gene in vitro.
FAU - Li, Hao
AU  - Li H
AD  - Department of General Surgery, Hunan People's Hospital, Changsha 410005, China.
FAU - Wu, Jinshu
AU  - Wu J
FAU - Jiang, Bo
AU  - Jiang B
FAU - Liang, Zhongshu
AU  - Liang Z
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.48 (HTATIP2 protein, human)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (MMP9 protein, human)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Acetyltransferases/*metabolism
MH  - Carcinoma, Hepatocellular/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Hep G2 Cells
MH  - Humans
MH  - Liver Neoplasms/*metabolism
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Matrix Metalloproteinase 9/metabolism
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis
MH  - Transcription Factors/*metabolism
MH  - Transfection
EDAT- 2014/09/10 06:00
MHDA- 2015/09/09 06:00
CRDT- 2014/09/10 06:00
PHST- 2014/09/10 06:00 [entrez]
PHST- 2014/09/10 06:00 [pubmed]
PHST- 2015/09/09 06:00 [medline]
AID - 10.3969/j.issn.1672-7347.2014.08.004 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2014 Aug;39(8):775-83. doi: 
      10.3969/j.issn.1672-7347.2014.08.004.