PMID- 25203430
OWN - NLM
STAT- MEDLINE
DCOM- 20151117
LR  - 20161020
IS  - 1533-4058 (Electronic)
IS  - 1533-4058 (Linking)
VI  - 23
IP  - 2
DP  - 2015 Feb
TI  - The HER2 gene and HER2 protein status and chromosome 17 polysomy in gastric 
      cancer cells in own material.
PG  - 113-7
LID - 10.1097/PAI.0000000000000070 [doi]
AB  - BACKGROUND: The aims of the study were to assess the expression of HER2 protein, 
      the amplification of the HER2 gene, and the occurrence of chromosome 17 polysomy 
      in gastric cancer cells and to analyze the relation between the results of such a 
      determination and the selected clinicopathologic parameters in patients treated 
      for gastric cancer. METHODS: Tissue samples of primary tumor from 83 consecutive 
      patients who underwent gastric cancer resection were analyzed by 
      immunohistochemical (IHC) analysis and fluorescence in situ hybridization (FISH). 
      RESULTS: A positive result of the IHC test, with a minimum score +, was obtained 
      among 22.8% patients. The FISH test was carried out successfully among 58 
      patients, including 10.3% cases with a positive result, whereas the presence of 
      chromosome 17 polysomy was confirmed among 13.8% patients. A statistically 
      significant dependence was found between the presence of HER2 overexpression and: 
      the lower stage of tumor infiltration, the higher grade of cancer 
      differentiation, no mucinous component, and the intestinal type according to the 
      Lauren classification. Statistically significant relation was found between 
      chromosome 17 polysomy and the tumor location in the proximal part of the 
      stomach, the performance of the palliative procedure, the presence of distant 
      metastases, and a higher frequency of postoperative complications. CONCLUSIONS: 
      There is no complete coincidence in gastric cancer between the occurrence of the 
      HER2 gene amplification and the HER2 receptor expression. The impact of the HER2 
      gene status and HER2 protein on prognosis in gastric cancer remains unclear. 
      Chromosome 17 polysomy may be an important negative prognostic factor in gastric 
      cancer.
FAU - Ciesielski, Maciej
AU  - Ciesielski M
AD  - *Department of Oncological Surgery double daggerDivision of Pathomorphology, Gdynia Oncology 
      Centre, Red Cross Maritime Hospital, Gdynia daggerDivision of Propedeutics of 
      Oncology, Medical University of Gdansk, Gdansk, Poland.
FAU - Kruszewski, Wieslaw Janusz
AU  - Kruszewski WJ
FAU - Smialek, Urszula
AU  - Smialek U
FAU - Walczak, Jakub
AU  - Walczak J
FAU - Szajewski, Mariusz
AU  - Szajewski M
FAU - Szefel, Jaroslaw
AU  - Szefel J
FAU - Wydra, Jacek
AU  - Wydra J
FAU - Kawecki, Krzysztof
AU  - Kawecki K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Immunohistochem Mol Morphol
JT  - Applied immunohistochemistry & molecular morphology : AIMM
JID - 100888796
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/metabolism
MH  - Chromosome Duplication
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Female
MH  - Gene Amplification
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Metastasis
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Receptor, ErbB-2/genetics/*metabolism
MH  - Stomach Neoplasms/*diagnosis/pathology
EDAT- 2014/09/10 06:00
MHDA- 2015/11/18 06:00
CRDT- 2014/09/10 06:00
PHST- 2014/09/10 06:00 [entrez]
PHST- 2014/09/10 06:00 [pubmed]
PHST- 2015/11/18 06:00 [medline]
AID - 10.1097/PAI.0000000000000070 [doi]
PST - ppublish
SO  - Appl Immunohistochem Mol Morphol. 2015 Feb;23(2):113-7. doi: 
      10.1097/PAI.0000000000000070.