PMID- 25203938 OWN - NLM STAT- MEDLINE DCOM- 20161213 LR - 20161230 IS - 1600-0765 (Electronic) IS - 0022-3484 (Linking) VI - 50 IP - 4 DP - 2015 Aug TI - Brain-derived neurotrophic factor prevents the endothelial barrier dysfunction induced by interleukin-1beta and tumor necrosis factor-alpha. PG - 444-51 LID - 10.1111/jre.12226 [doi] AB - BACKGROUND AND OBJECTIVE: Brain-derived neurotrophic factor (BDNF) promotes the regeneration of periodontal tissue. Although a local inflammatory step is required to initiate the subsequent process of tissue regeneration, excessive inflammation may inhibit or delay tissue regeneration. Therefore, the regulation of inflammation is essential for periodontal tissue regeneration. In the present study, we examined the influence of BDNF on the human microvascular endothelial cell (HMVEC) barrier dysfunction induced by interleukin (IL)-1beta or tumor necrosis factor (TNF)-alpha to determine the effects of BDNF on the regulation of local inflammation in periodontal tissue regeneration. MATERIAL AND METHODS: Endothelial permeability was analyzed using a Dextran transport assay with transwell plates. The expression of vascular endothelial (VE)-cadherin was assessed by immunoblotting and immunofluorescence microscopy. RESULTS: BDNF (25 ng/mL) inhibited increase induced in endothelial permeability by IL-1beta and TNF-alpha. IL-1beta and TNF-alpha decreased VE-cadherin protein levels, while BDNF recovered the reduction in HMVECs. BDNF protected the increase induced in endothelial permeability by IL-1beta and TNF-alpha through TrkB. The single addition of BDNF into the culture increased the expression of VE-cadherin in HMVECs. CONCLUSION: BDNF played an important role in inhibiting endothelial barrier dysfunction, which suggests that it may assist in enhancing periodontal tissue regeneration. CI - (c) 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. FAU - Matsuda, S AU - Matsuda S AD - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Fujita, T AU - Fujita T AD - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Kajiya, M AU - Kajiya M AD - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Kashiwai, K AU - Kashiwai K AD - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Takeda, K AU - Takeda K AD - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Shiba, H AU - Shiba H AD - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Kurihara, H AU - Kurihara H AD - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140909 PL - United States TA - J Periodontal Res JT - Journal of periodontal research JID - 0055107 RN - 0 (Antigens, CD) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Cadherins) RN - 0 (Carbazoles) RN - 0 (Dextrans) RN - 0 (Enzyme Inhibitors) RN - 0 (Fluorescent Dyes) RN - 0 (Indole Alkaloids) RN - 0 (Interleukin-1beta) RN - 0 (Tumor Necrosis Factor-alpha) RN - 0 (cadherin 5) RN - 0 (fluorescein isothiocyanate dextran) RN - 97161-97-2 (staurosporine aglycone) RN - EC 2.7.10.1 (Receptor, trkB) RN - I223NX31W9 (Fluorescein-5-isothiocyanate) SB - IM MH - Antigens, CD/drug effects MH - Brain-Derived Neurotrophic Factor/*pharmacology MH - Cadherins/drug effects MH - Carbazoles/pharmacology MH - Cell Line MH - Cell Membrane Permeability/drug effects MH - Dextrans MH - Endothelial Cells/*drug effects MH - Endothelium, Vascular/*drug effects MH - Enzyme Inhibitors/pharmacology MH - Fluorescein-5-isothiocyanate/analogs & derivatives MH - Fluorescent Dyes MH - Humans MH - Immunoblotting MH - Indole Alkaloids/pharmacology MH - Interleukin-1beta/*pharmacology MH - Microscopy, Fluorescence MH - Receptor, trkB/antagonists & inhibitors MH - Tumor Necrosis Factor-alpha/*pharmacology OTO - NOTNLM OT - brain-derived neurotrophic factor OT - endothelial cell permeability OT - inflammatory cytokine OT - periodontal tissue regeneration EDAT- 2014/09/10 06:00 MHDA- 2016/12/15 06:00 CRDT- 2014/09/10 06:00 PHST- 2014/07/13 00:00 [accepted] PHST- 2014/09/10 06:00 [entrez] PHST- 2014/09/10 06:00 [pubmed] PHST- 2016/12/15 06:00 [medline] AID - 10.1111/jre.12226 [doi] PST - ppublish SO - J Periodontal Res. 2015 Aug;50(4):444-51. doi: 10.1111/jre.12226. Epub 2014 Sep 9.