PMID- 25204040
OWN - NLM
STAT- MEDLINE
DCOM- 20140930
LR  - 20161021
IS  - 1110-4902 (Print)
VI  - 21
IP  - 1
DP  - 2014
TI  - Interleukin-18 gene polymorphisms in systemic lupus erythematosus: relation to 
      disease status.
PG  - 1-12
AB  - Systemic lupus erythematosus (SLE) is a multisystem autoimmune connective tissue 
      disorder characterized by loss of self-tolerance causing immune-mediated tissue 
      destruction and various clinical presentations Interleukin-18 (IL-18) is a 
      proinflammatory cytokine that plays an important role in chronic inflammation and 
      autoimmune disorders. This study investigates polymorphisms of the IL-18 gene in 
      SLE patients at positions -607 and -137 of the promoter to elucidate their 
      possible roles in the activity and severity of this disease. Fifty SLE patients 
      and fifty unrelated healthy control group were included. AII SLE patients 
      underwent thorough clinical examination and SLE disease activity assessment using 
      SLEDAI. Genomic DNA was extracted from peripheral venous blood. Sequence-specific 
      primer PCR analysis were used to genotype the DNA samples for SNP-607and SNP-137, 
      while plasma IL-18 concentrations of patients and control subjects were measured 
      by enzyme-linked immunosorbent assay. The frequency of SNP-607/CC genotype showed 
      significant increase (P < 0.05), while genotype SNP-607/CA showed significant 
      decrease (P < 0.05) in SLE patients as compared to the control group. 
      Significantly elevated levels of plasma IL-18 were found in patients compared to 
      controls (P < 0.001) and those with genotype CC at -607 demonstrated the highest 
      IL-18 level (331.74 +/- 36.48 pg/mL). Serum IL-18 levels showed significant 
      positive correlations with the ESR 1st hr. (r = 0.89), protein/creatinine ratios 
      (r = 0.88), anti-dsDNA titers (r = 0.44) and SLEDAI scores (r = 0.91). In 
      contrast, significant negative correlations were found with HB% r = -0.68, 
      creatinine clearance (r = -0.87) and C3 (r = -0.81). In addition, a statistically 
      significant association was found between IL-18 of CC -607 genotype and lupus 
      nephritis, arthritis and immunological disorders. In conclusion, IL-18 promoter 
      gene polymorphisms at position -607 may contribute to SLE activity and accelerate 
      SLE development by enhancing production of IL-18 protein in SLE patients.
FAU - Fouad, Nehad A
AU  - Fouad NA
FAU - Baraka, Eman A
AU  - Baraka EA
FAU - Hassan, Waleed A
AU  - Hassan WA
LA  - eng
PT  - Journal Article
PL  - Egypt
TA  - Egypt J Immunol
JT  - The Egyptian journal of immunology
JID - 9816016
RN  - 0 (Interleukin-18)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Alleles
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Gene Frequency
MH  - Genotype
MH  - Humans
MH  - Interleukin-18/blood/*genetics
MH  - Lupus Erythematosus, Systemic/blood/*genetics/pathology
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Single Nucleotide
MH  - Promoter Regions, Genetic/*genetics
MH  - Severity of Illness Index
MH  - Young Adult
EDAT- 2014/09/11 06:00
MHDA- 2014/10/01 06:00
CRDT- 2014/09/11 06:00
PHST- 2014/09/11 06:00 [entrez]
PHST- 2014/09/11 06:00 [pubmed]
PHST- 2014/10/01 06:00 [medline]
PST - ppublish
SO  - Egypt J Immunol. 2014;21(1):1-12.