PMID- 25204183
OWN - NLM
STAT- MEDLINE
DCOM- 20140930
LR  - 20140910
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 39
IP  - 5
DP  - 2014 Mar
TI  - [Study on anti-tumor and anti-metastasis mechanism of alcohol extracts from 
      pharbitidis semen against Lewis lung cancer].
PG  - 879-84
AB  - OBJECTIVE: To observe the effect of alcohol extracts from Pharbitidis Semen on 
      the proliferation and metastasis of Lewis lung cancer, and study its anti-tumor 
      mechanism. METHOD: In vitro, MTT assay and scratch assay were adopted to detect 
      the effect of alcohol extracts from Pharbitidis Semen on the proliferation and 
      metastasis of Lewis lung cancer cells. The cell autophagy was detected by the 
      acridine orange staining. The gap-junction intercellular communication (GJIC) was 
      investigated by the fluorescent yellow transfer. The expression of aquaporin 1 
      (AQP1) was analyzed by the Western blotting. In vivo, the subcutaneous implant 
      model and the experimental pulmonary metastasis model of Lewis lung cancer in 
      mice were established to evaluate the anti-tumor and anti-metastasis effects of 
      alcohol extract from Pharbitidis Semen. The serum carcinoembryonic antigen (CEA) 
      and beta2 microglobulin (beta2-MG) of mice bearing Lewis lung cancer were 
      detected by the electrochemiluminesence immunoassay. The expressions of lung AQP1 
      and Connexin 43 (Cx43) were examined by the immunohistochemical method. RESULT: 
      In vitro, alcohol extracts from Pharbitidis Semen inhibited the cell 
      proliferation in a dose-dependent matter, significantly prevented the cell 
      migration, down-regulated AQP1 proteins of cells, promoted GJIC, and decreased 
      the serum-free autophagy of tumor cells. In vivo, compared with untreated model 
      mice, alcohol extracts from Pharbitidis Semen inhibited the tumor growth in a 
      dose-dependent matter, prevented the tumor metastasis and prolonged the life span 
      of mice bearing Lewis lung cancer, while decreasing serum CEA and beta2-MG of 
      mice bearing Lewis lung cancer, enhancing the immumohistochemical staining 
      intensity of Cx43 and weakening aquaporins AQP1 positive intensity. CONCLUSION: 
      Alcohol extracts from Pharbitidis Semen could prevent the proliferation and 
      metastasis in Lewis lung cancer cells. Its mechanism may be related to the 
      promotion of GJIC and the down-regulation of AQP1.
FAU - Li, Jia-Huan
AU  - Li JH
FAU - Du, Gang-Jun
AU  - Du GJ
FAU - Liu, Wei-Jie
AU  - Liu WJ
FAU - Liu, Ying-Hui
AU  - Liu YH
FAU - Zhao, Bei
AU  - Zhao B
FAU - Li, Hong
AU  - Li H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Connexin 43)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 146410-94-8 (Aquaporin 1)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*administration & dosage
MH  - Aquaporin 1/genetics/metabolism
MH  - Carcinoma, Lewis Lung/*drug therapy/genetics/metabolism/pathology
MH  - Cell Line, Tumor
MH  - Connexin 43/genetics/metabolism
MH  - Disease Models, Animal
MH  - Drugs, Chinese Herbal/*administration & dosage
MH  - Humans
MH  - Ipomoea/*chemistry
MH  - Lung Neoplasms/*drug therapy/genetics/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neoplasm Metastasis
MH  - Seeds/*chemistry
EDAT- 2014/09/11 06:00
MHDA- 2014/10/01 06:00
CRDT- 2014/09/11 06:00
PHST- 2014/09/11 06:00 [entrez]
PHST- 2014/09/11 06:00 [pubmed]
PHST- 2014/10/01 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2014 Mar;39(5):879-84.