PMID- 25204185
OWN - NLM
STAT- MEDLINE
DCOM- 20140930
LR  - 20140910
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 39
IP  - 5
DP  - 2014 Mar
TI  - [Study on effect of huatuo zaizao extractum on focal cerebral 
      ischemia/reperfusion neurogenesis in rats and its mechanisms].
PG  - 891-5
AB  - OBJECTIVE: To observe the effect of Huatuo Zaizao extractum (HTZZ) on focal 
      cerebral ischemia/reperfusion (I/R) neurogenesis in rats induced by middle 
      cerebral artery occlusion (MCAO) and its mechanism. METHOD: Totally 55 healthy 
      adult male Sprague-Dawley rats were divided into the sham operation group, the 
      MCAO model group and HTZZ high, middle and low dose groups (5, 2.5, 1.25 g x 
      kg(-1)), with 11 rats in each group, and orally administered with drugs. The 
      focal cerebral ischemia model was established by performing a middle cerebral 
      artery occlusion (MCAO, 90 min) followed by a seven-day reperfusion (once a day). 
      The neurogenesis and expressions of extracellular signal-regulated kinase (ERK) 
      and cAMP response element binding protein (CREB) were detected by the 
      immunofluorescent staining. The enzyme linked immunosorbent assay (ELISA) was 
      adopted to determine the vascular endothelial growth factor (VEGF) and 
      brain-derived neurotrophic factor (BDNF). RESULT: MCAO (90 min) followed by a 
      seven-day reperfusion resulted in the significant increase in the number of 
      penumbra cortex newborn neurons (BrdU(+) -NeuN(+)), which was accompanied by the 
      growth of ERK and CREB phosphorylation and VEGF and BDNF levels. HTZZ could 
      promote the generation of newborn neurons (BrdU(+)-NeuN(+)) and the ERK and CREB 
      phosphorylation and increase VEGF and BDNF levels at the ischemic side. 
      CONCLUSION: HTZZ could promote the neurogenesis, which may be the interventional 
      targets of effective traditional Chinese medicine Huatuo Zaizao extractum in 
      promoting the self-repair function of the cerebral ischemic areas.
FAU - Zheng, Yong-Qiu
AU  - Zheng YQ
FAU - Li, Lei
AU  - Li L
FAU - Liu, Jian-Xun
AU  - Liu JX
FAU - Yao, Ming-Jiang
AU  - Yao MJ
FAU - Liu, Sheng-Bo
AU  - Liu SB
FAU - Hu, Yan
AU  - Hu Y
FAU - Si, Jing-Xi
AU  - Si JX
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/*drug therapy/genetics/metabolism/physiopathology
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Drugs, Chinese Herbal/*administration & dosage
MH  - Humans
MH  - Male
MH  - Neurogenesis/drug effects
MH  - Neurons/*cytology/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
EDAT- 2014/09/11 06:00
MHDA- 2014/10/01 06:00
CRDT- 2014/09/11 06:00
PHST- 2014/09/11 06:00 [entrez]
PHST- 2014/09/11 06:00 [pubmed]
PHST- 2014/10/01 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2014 Mar;39(5):891-5.