PMID- 25204351
OWN - NLM
STAT- MEDLINE
DCOM- 20160331
LR  - 20220321
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 90
IP  - 3
DP  - 2014 Dec
TI  - Halophyte plant colonization as a driver of the composition of bacterial 
      communities in salt marshes chronically exposed to oil hydrocarbons.
PG  - 647-62
LID - 10.1111/1574-6941.12425 [doi]
AB  - In this study, two molecular techniques [denaturing gradient gel electrophoresis 
      (DGGE) and barcoded pyrosequencing] were used to evaluate the composition of 
      bacterial communities in salt marsh microhabitats [bulk sediment and sediment 
      surrounding the roots (rhizosphere) of Halimione portulacoides and Sarcocornia 
      perennis ssp. perennis] that have been differentially affected by oil hydrocarbon 
      (OH) pollution. Both DGGE and pyrosequencing revealed that bacterial composition 
      is structured by microhabitat. Rhizosphere sediment from both plant species 
      revealed enrichment of operational taxonomic units closely related to 
      Acidimicrobiales, Myxococcales and Sphingomonadales. The in silico metagenome 
      analyses suggest that homologous genes related to OH degradation appeared to be 
      more frequent in both plant rhizospheres than in bulk sediment. In summary, this 
      study suggests that halophyte plant colonization is an important driver of 
      hydrocarbonoclastic bacterial community composition in estuarine environments, 
      which can be exploited for in situ phytoremediation of OH in salt marsh 
      environments.
CI  - (c) 2014 Federation of European Microbiological Societies. Published by John Wiley 
      & Sons Ltd. All rights reserved.
FAU - Oliveira, Vanessa
AU  - Oliveira V
AD  - Department of Biology & Centre for Environmental and Marine Studies (CESAM), 
      University of Aveiro, Campus Universitario de Santiago, Aveiro, Portugal.
FAU - Gomes, Newton C M
AU  - Gomes NC
FAU - Cleary, Daniel F R
AU  - Cleary DF
FAU - Almeida, Adelaide
AU  - Almeida A
FAU - Silva, Artur M S
AU  - Silva AM
FAU - Simoes, Mario M Q
AU  - Simoes MM
FAU - Silva, Helena
AU  - Silva H
FAU - Cunha, Angela
AU  - Cunha A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141002
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (Hydrocarbons)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Alphaproteobacteria/classification/metabolism
MH  - Amaranthaceae/microbiology/physiology
MH  - Bacteria/*classification/genetics/isolation & purification
MH  - Base Sequence
MH  - Biodegradation, Environmental
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Geologic Sediments/microbiology
MH  - Hydrocarbons/*metabolism
MH  - Metagenome/genetics
MH  - Microbial Consortia/genetics
MH  - *Petroleum Pollution
MH  - Plant Roots/*microbiology
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Rhizosphere
MH  - Salt-Tolerant Plants/microbiology/*physiology
MH  - Sequence Analysis, DNA
MH  - *Wetlands
OTO - NOTNLM
OT  - microbial diversity
OT  - pyrosequencing
OT  - rhizosphere
OT  - salt marshes
EDAT- 2014/09/11 06:00
MHDA- 2016/04/01 06:00
CRDT- 2014/09/11 06:00
PHST- 2014/05/13 00:00 [received]
PHST- 2014/08/08 00:00 [revised]
PHST- 2014/08/31 00:00 [accepted]
PHST- 2014/09/11 06:00 [entrez]
PHST- 2014/09/11 06:00 [pubmed]
PHST- 2016/04/01 06:00 [medline]
AID - 10.1111/1574-6941.12425 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2014 Dec;90(3):647-62. doi: 10.1111/1574-6941.12425. Epub 
      2014 Oct 2.