PMID- 25208991
OWN - NLM
STAT- MEDLINE
DCOM- 20150411
LR  - 20160818
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 43
IP  - 6
DP  - 2014 Jun
TI  - [Construction of a repeat-free dual color fluorescent in situ hybridization probe 
      for ROS1 gene in non-small cell lung cancer diagnosis].
PG  - 399-402
AB  - OBJECTIVE: To establish a repeat-free ROS1 gene fluorescence in situ 
      hybridization (FISH) probe, and to compare its efficacy with those of commercial 
      FISH probes in non-small cell lung cancer. METHODS: The probe was constructed by 
      combining human Cot-1 DNA genome into double-stranded sequence, and then digested 
      by duples specific nuclease to establish a repeat-free sequence. The final 
      repeat-free ROS1 FISH probe was labeled by red and green fluoresceins. RESULTS: 
      Compared with the commercialized probe, repeat-free FISH probe exhibited 
      excellent efficiency and low signal to noise ratio (SNR) in samples. There was 
      statistical significance in the difference between the hybridization rate of 
      these two probes (P < 0.05) , but there was no difference between the accuracy 
      rate (P > 0.05). CONCLUSION: The repeat-free ROS1 FISH probe significantly 
      improves the probe hybridization efficiency and SNR in non-small cell lung cancer 
      (NSCLC), resulting in an increased accuracy of detection.
FAU - Cheng, Hongxia
AU  - Cheng H
AD  - Huaxia College, Wuhan University of Technology, Wuhan 430223, China.
FAU - Ye, Lun
AU  - Ye L
FAU - Xue, Liquan
AU  - Xue L
AD  - Email: healthchart@163.com.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (ROS1 protein, human)
SB  - IM
MH  - Carcinoma, Non-Small-Cell Lung/*diagnosis/genetics
MH  - *Fluorescent Dyes
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Protein-Tyrosine Kinases/*genetics
MH  - Proto-Oncogene Proteins/*genetics
EDAT- 2014/09/12 06:00
MHDA- 2015/04/12 06:00
CRDT- 2014/09/12 06:00
PHST- 2014/09/12 06:00 [entrez]
PHST- 2014/09/12 06:00 [pubmed]
PHST- 2015/04/12 06:00 [medline]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2014 Jun;43(6):399-402.