PMID- 25209151
OWN - NLM
STAT- MEDLINE
DCOM- 20150629
LR  - 20141021
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 84
IP  - 5
DP  - 2014 Nov
TI  - Resolution of HLA-B*44:02:01G, -DRB1*14:01:01G and -DQB1*03:01:01G reveals a high 
      allelic variability among 12 European populations.
PG  - 459-64
LID - 10.1111/tan.12422 [doi]
AB  - Within the framework of the EU-funded HLA-NET action, an analysis of three 
      G-group alleles, HLA-B*44:02:01G, DRB1*14:01:01G and DQB1*03:01:01G, was 
      undertaken in 12 European populations. Ambiguities were resolved by polymerase 
      chain reaction-sequence-specific amplification (PCR-SSP) or PCR-sequence-based 
      typing (PCR-SBT) in a total of 5095 individuals. The results of the 
      DRB1*14:01/14:54 ambiguity showed high relative ratios (24-53%) of DRB1*14:01 in 
      Bulgarians, Croatians, Greeks, Italians and Slovenians, contrasting with low 
      ratios (6-13%) in Austrians, Finnish, French, Hungarians, Norwegians and Swiss. 
      Resolution of the B*44:02/44:27 ambiguity showed that B*44:27 had a high relative 
      ratio in Slovenians (25.5%) and Bulgarians (37%) and low in French and Swiss 
      (0.02-1%), and was not observed in Greeks and Italians. The highest relative 
      ratio of DQB1*03:19 was found in Portuguese (11%), by contrast with low ratios 
      (0-3%) in the other five populations. Analysis of the A, B, DRB1 phenotypes and 
      family-derived haplotypes in 1719 and 403 individuals positive for either 
      HLA-B*44:02G or DRB1*14:01G ambiguities, respectively, showed some preferential 
      associations, such as A*26 approximately DRB1*14:01, B*35 approximately DRB1*14:01, B*38 approximately DRB1*14:01 and 
      B*44:27 approximately DRB1*16. Because these ambiguities are located outside the 
      peptide-binding site, they may not be recognized by alloreactive T-cells. 
      However, because of strong linkage disequilibrium (LD), the DRB1*14:01 vs 
      DRB1*14:54 and the B*44:02 vs B*44:27 mismatches are associated to DRB3-, and 
      C-mismatches, respectively. These results are informative for algorithms 
      searching unrelated hematopoietic stem cell donors. For B*44:27-positive 
      patients, searches are expected to be more successful when requesting donors from 
      Southeastern-European ancestry. Furthermore, the introduction of human leukocyte 
      antigen (HLA)-typing strategies that allow resolving exon 4 (for class I) and 
      exon 3 (for class II) polymorphisms can be expected to contribute significantly 
      to population genetics studies.
CI  - (c) 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Vidan-Jeras, B
AU  - Vidan-Jeras B
AD  - Tissue Typing Center, Blood Transfusion Center of Slovenia, Ljubljana, Slovenia.
FAU - Buhler, S
AU  - Buhler S
FAU - Dubois, V
AU  - Dubois V
FAU - Grubic, Z
AU  - Grubic Z
FAU - Ivanova, M
AU  - Ivanova M
FAU - Jaatinen, T
AU  - Jaatinen T
FAU - Ligeiro, D
AU  - Ligeiro D
FAU - Lokki, M-L
AU  - Lokki ML
FAU - Papasteriades, C
AU  - Papasteriades C
FAU - Poli, F
AU  - Poli F
FAU - Spyropoulou-Vlachou, M
AU  - Spyropoulou-Vlachou M
FAU - Tordai, A
AU  - Tordai A
FAU - Viken, M K
AU  - Viken MK
FAU - Wenda, S
AU  - Wenda S
FAU - Nunes, J M
AU  - Nunes JM
FAU - Sanchez-Mazas, A
AU  - Sanchez-Mazas A
FAU - Tiercy, J-M
AU  - Tiercy JM
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20140911
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (HLA-DRB1*03:01 antigen)
SB  - IM
MH  - *Alleles
MH  - Donor Selection
MH  - Europe
MH  - Female
MH  - *Gene Frequency
MH  - *Genetic Variation
MH  - HLA-B Antigens/*genetics
MH  - HLA-DRB1 Chains/*genetics
MH  - Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Living Donors
MH  - Male
OTO - NOTNLM
OT  - B*44:02/44:27
OT  - DRB1*14:01/14:54
OT  - European populations
OT  - human leukocyte antigen ambiguities
OT  - human leukocyte antigen incompatibilities
EDAT- 2014/09/12 06:00
MHDA- 2015/06/30 06:00
CRDT- 2014/09/12 06:00
PHST- 2014/05/16 00:00 [received]
PHST- 2014/07/14 00:00 [revised]
PHST- 2014/07/16 00:00 [accepted]
PHST- 2014/09/12 06:00 [entrez]
PHST- 2014/09/12 06:00 [pubmed]
PHST- 2015/06/30 06:00 [medline]
AID - 10.1111/tan.12422 [doi]
PST - ppublish
SO  - Tissue Antigens. 2014 Nov;84(5):459-64. doi: 10.1111/tan.12422. Epub 2014 Sep 11.