PMID- 25209714
OWN - NLM
STAT- MEDLINE
DCOM- 20160314
LR  - 20220316
IS  - 1942-7611 (Electronic)
IS  - 1942-7603 (Linking)
VI  - 7
IP  - 6
DP  - 2015 Jun
TI  - Histochemical evaluation of alkaloids in rhizome of Coptis chinensis using laser 
      microdissection and liquid chromatography/mass spectrometry.
PG  - 519-30
LID - 10.1002/dta.1703 [doi]
AB  - Traditional macroscopic and microscopic identification methods of medicinal 
      materials are economical and practical, but usually experience-based due to few 
      chemical supports. Here histochemical evaluation on bioactive components of 
      Coptidis Rhizoma (CR) in anatomic sections using laser microdissection and liquid 
      chromatography-mass spectrometry (LMD-LC-MS) was developed to correlate the inner 
      quality and outer features of materials from different growing areas. Results of 
      a total 33 peaks representing potential different alkaloids were detected and 8 
      common peaks were identified as the major alkaloids, namely magnoflorine, 
      thalifendine, columbamine, epiberberine, jatrorrhizine, coptisine, palmatine, and 
      berberine. Six major alkaloids were quantified in the top and middle sections of 
      raw materials and in their tissues and cells at the same time. Histochemical 
      analyses showed consistent results with direct determination in raw materials and 
      explained the reason why top sections of all samples contained higher contents of 
      alkaloids by giving out attributions of each alkaloid in different anatomic 
      sections. Besides, results manifested the distribution and accumulation rules of 
      alkaloids in diverse tissues and cells of CR. This study demonstrates an 
      effective and scientific way to correlate bioactive components and morphological 
      features of medicinal materials, which is beneficial to future research, 
      agriculture and application.
CI  - Copyright (c) 2014 John Wiley & Sons, Ltd.
FAU - Yi, Ling
AU  - Yi L
AD  - School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, 
      Hong Kong, People's Republic of China.
AD  - Department of Pharmaceutics, College of Pharmacy, Ohio State University, 
      Columbus, OH, USA.
FAU - Liang, Zhi-Tao
AU  - Liang ZT
AD  - School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, 
      Hong Kong, People's Republic of China.
FAU - Peng, Yong
AU  - Peng Y
AD  - Institute of Medicinal Plant Development, Peking Union Medical College & Chinese 
      Academy of Medical Sciences, Haidian District, Beijing, People's Republic of 
      China.
FAU - Guo, Ping
AU  - Guo P
AD  - School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, 
      Hong Kong, People's Republic of China.
FAU - Wong, Lai-Lai
AU  - Wong LL
AD  - School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, 
      Hong Kong, People's Republic of China.
FAU - Wan, Xiao-Jing
AU  - Wan XJ
AD  - School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, 
      Hong Kong, People's Republic of China.
FAU - Ho, Hing-Man
AU  - Ho HM
AD  - School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, 
      Hong Kong, People's Republic of China.
FAU - Yi, Tao
AU  - Yi T
AD  - School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, 
      Hong Kong, People's Republic of China.
FAU - Zhao, Zhong-Zhen
AU  - Zhao ZZ
AUID- ORCID: 0000-0002-6533-9668
AD  - School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, 
      Hong Kong, People's Republic of China.
AD  - Institute of Medicinal Plant Development, Peking Union Medical College & Chinese 
      Academy of Medical Sciences, Haidian District, Beijing, People's Republic of 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140910
PL  - England
TA  - Drug Test Anal
JT  - Drug testing and analysis
JID - 101483449
RN  - 0 (Alkaloids)
RN  - 0 (Drugs, Chinese Herbal)
SB  - IM
MH  - Alkaloids/*analysis
MH  - Chromatography, Liquid
MH  - Coptis/*anatomy & histology/*chemistry
MH  - Drugs, Chinese Herbal/chemistry
MH  - Histological Techniques/*methods
MH  - *Laser Capture Microdissection
MH  - Mass Spectrometry
MH  - Rhizome/*anatomy & histology/*chemistry
OTO - NOTNLM
OT  - Coptidis Rhizoma
OT  - histochemistry
OT  - laser microdissection and liquid chromatography-mass spectrometry
OT  - quality evaluation
EDAT- 2014/09/12 06:00
MHDA- 2016/03/15 06:00
CRDT- 2014/09/12 06:00
PHST- 2014/06/12 00:00 [received]
PHST- 2014/07/15 00:00 [revised]
PHST- 2014/07/18 00:00 [accepted]
PHST- 2014/09/12 06:00 [entrez]
PHST- 2014/09/12 06:00 [pubmed]
PHST- 2016/03/15 06:00 [medline]
AID - 10.1002/dta.1703 [doi]
PST - ppublish
SO  - Drug Test Anal. 2015 Jun;7(6):519-30. doi: 10.1002/dta.1703. Epub 2014 Sep 10.