PMID- 25209829
OWN - NLM
STAT- MEDLINE
DCOM- 20150821
LR  - 20181202
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 116
IP  - 2
DP  - 2015 Feb
TI  - Human umbilical cord mesenchymal stem cells alleviate nasal mucosa radiation 
      damage in a guinea pig model.
PG  - 331-8
LID - 10.1002/jcb.24975 [doi]
AB  - Nasal complications after radiotherapy severely affect the quality of life of 
      nasopharyngeal carcinoma patients, and there is a compelling need to find novel 
      therapies for nasal epithelial cell radiation damage. Therefore, we investigated 
      the therapeutic effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) 
      in guinea pig model of nasal mucosa radiation damage and explored its therapeutic 
      mechanism. Cultured hUC-MSCs were injected intravenously immediately after 
      radiation in the nasal mucosa-radiation-damage guinea pig model. Migration of 
      hUC-MSCs into the nasal mucosa and the potential for differentiation into nasal 
      epithelial cells were evaluated by immunofluorescence. The therapeutic effects of 
      hUC-MSCs were evaluated by mucus clearance time (MCT), degree of nasal mucosa 
      edema, and the nasal mucosa cilia form and coverage ratio. Results indicate that 
      the hUC-MSCs migrated to the nasal mucosa lamina propria and did not 
      differentiate into nasal epithelial cells in this model. The MCT and degree of 
      mucosal edema were improved at 1 week and 1 month after radiation, respectively, 
      but no difference was found at 3 months and 6 months after radiation. The nasal 
      mucosa cilia form and coverage ratio was not improved 6 months after radiation. 
      Thus, hUC-MSCs can migrate to the nasal mucosa lamina propria and improve MCT and 
      mucosa edema within a short time period, but these cells are unable to 
      differentiate into nasal epithelial cells and improve nasal epithelial 
      regeneration in the nasal mucosa radiation damage guinea pig model.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Duan, Hong-Gang
AU  - Duan HG
AD  - Department of Otolaryngology, Second Affiliated Hospital, College of Medicine, 
      Zhejiang University, Hangzhou, China.
FAU - Ji, Fang
AU  - Ji F
FAU - Zheng, Chun-Quan
AU  - Zheng CQ
FAU - Wang, Chun-Hua
AU  - Wang CH
FAU - Li, Jing
AU  - Li J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Cilia/physiology/radiation effects
MH  - Edema/therapy
MH  - Flow Cytometry
MH  - Guinea Pigs
MH  - Humans
MH  - Immunophenotyping
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Nasal Mucosa/*diagnostic imaging/pathology/physiopathology
MH  - Radiation Injuries, Experimental/*therapy
MH  - Radiography
MH  - Transplantation, Heterologous
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - MESENCHYMAL STEM CELLS
OT  - NASOPHARYNGEAL CARCINOMA
OT  - RADIATION DAMAGE
OT  - STEM CELL TRANSPLANTATION
EDAT- 2014/09/12 06:00
MHDA- 2015/08/22 06:00
CRDT- 2014/09/12 06:00
PHST- 2014/06/15 00:00 [received]
PHST- 2014/09/05 00:00 [accepted]
PHST- 2014/09/12 06:00 [entrez]
PHST- 2014/09/12 06:00 [pubmed]
PHST- 2015/08/22 06:00 [medline]
AID - 10.1002/jcb.24975 [doi]
PST - ppublish
SO  - J Cell Biochem. 2015 Feb;116(2):331-8. doi: 10.1002/jcb.24975.