PMID- 25215505
OWN - NLM
STAT- MEDLINE
DCOM- 20151215
LR  - 20220409
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 9
DP  - 2014
TI  - Excretion of urinary orosomucoid 1 protein is elevated in patients with chronic 
      heart failure.
PG  - e107550
LID - 10.1371/journal.pone.0107550 [doi]
LID - e107550
AB  - Easily screening markers for early detection of chronic heart failure (CHF) are 
      lacking. We identified twenty differently expressed proteins including 
      orosomucoid 1(ORM1) in urine between patients with CHF and normal controls by 
      proteomic methods. Bioinformatics analyses suggested ORM1 could be used for 
      further analysis. After verification by western blotting, the urinary levels of 
      ORM1 were quantified with enzyme-linked immunosorbent assay (ELISA) by correcting 
      for creatinine expression. The ORM1-Cr was significantly elevated in CHF patients 
      than normal controls (6498.83 +/- 4300.21 versus 2102.26 +/- 1069.24 ng/mg). 
      Furthermore, a Spearman analysis indicated that the urinary ORM1 levels had a 
      high positive correlation with the classification of CHF, and the multivariate 
      analysis suggested that the urinary ORM1 content was associated with the plasma 
      amino-terminal pro- brain natriuretic peptide (NT-proBNP) (OR: 2.106, 95% CI: 
      1.213-3.524, P = 0.002) and the New York Heart Association (NYHA) classification 
      (OR: 3.019, 95% CI: 1.329-4.721, P<0.001). In addition, receiving operating curve 
      (ROC) analyses suggested that an optimum cut-off value of 2484.98 ng/mg with 
      90.91% sensitivity and 85.48% specificity, respectively, could be used for the 
      diagnosis of CHF. To sum up, our findings indicate that ORM1 could be a potential 
      novel urinary biomarker for the early detection of CHF.
FAU - Hou, Li-na
AU  - Hou LN
AD  - State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang 
      Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China; 
      Department of healthy management, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong, P.R.China.
FAU - Li, Fei
AU  - Li F
AD  - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, 
      Guangdong, P.R.China.
FAU - Zeng, Qing-chun
AU  - Zeng QC
AD  - State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang 
      Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
FAU - Su, Liang
AU  - Su L
AD  - State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang 
      Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
FAU - Chen, Ping-an
AU  - Chen PA
AD  - State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang 
      Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
FAU - Xu, Zhi-hao
AU  - Xu ZH
AD  - State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang 
      Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
FAU - Zhu, Din-ji
AU  - Zhu DJ
AD  - State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang 
      Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
FAU - Liu, Chang-hua
AU  - Liu CH
AD  - State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang 
      Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
FAU - Xu, Ding-li
AU  - Xu DL
AD  - State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang 
      Hospital, Southern Medical University, Guangzhou, Guangdong, P.R.China.
LA  - eng
PT  - Journal Article
DEP - 20140912
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Biomarkers)
RN  - 0 (Orosomucoid)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers/*urine
MH  - Chronic Disease
MH  - Creatinine/urine
MH  - Female
MH  - Heart Failure/*urine
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Orosomucoid/*urine
PMC - PMC4162620
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/09/13 06:00
MHDA- 2015/12/17 06:00
PMCR- 2014/09/12
CRDT- 2014/09/13 06:00
PHST- 2014/02/26 00:00 [received]
PHST- 2014/08/12 00:00 [accepted]
PHST- 2014/09/13 06:00 [entrez]
PHST- 2014/09/13 06:00 [pubmed]
PHST- 2015/12/17 06:00 [medline]
PHST- 2014/09/12 00:00 [pmc-release]
AID - PONE-D-14-08815 [pii]
AID - 10.1371/journal.pone.0107550 [doi]
PST - epublish
SO  - PLoS One. 2014 Sep 12;9(9):e107550. doi: 10.1371/journal.pone.0107550. 
      eCollection 2014.