PMID- 25218480
OWN - NLM
STAT- MEDLINE
DCOM- 20150723
LR  - 20240329
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 195
DP  - 2015 Jan 2
TI  - Host miR-26a suppresses replication of porcine reproductive and respiratory 
      syndrome virus by upregulating type I interferons.
PG  - 86-94
LID - S0168-1702(14)00340-2 [pii]
LID - 10.1016/j.virusres.2014.08.012 [doi]
AB  - MicroRNAs (miRNAs) play important roles in viral infections, especially by 
      modulating the expression of cellular factors essential to viral replication or 
      the host innate immune response to infection. To identify host miRNAs important 
      to controlling porcine reproductive and respiratory syndrome virus (PRRSV) 
      infection, we screened 15 miRNAs that were previously implicated in innate 
      immunity or antiviral functions. Over-expression of the miR-26 family strongly 
      inhibited PRRSV replication in vitro, as shown by virus titer assays, Western 
      blotting, and qRT-PCR assays. MiR-26a inhibited the replication of both type 1 
      and type 2 PRRSV strains. Mutating the seed region of miR-26 restored viral 
      titers. Luciferase reporters showed that miR-26a does not target the PRRSV genome 
      directly but instead affects the expression of type I interferon and the 
      IFN-stimulated genes MX1 and ISG15 during PRRSV infection. These results 
      demonstrate the important role of miR-26a in modulating PRRSV infection and also 
      support the possibility of using host miR-26a to achieve RNAi-mediated antiviral 
      therapeutic strategies.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Li, Liwei
AU  - Li L
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Wei, Zuzhang
AU  - Wei Z
AD  - College of Animal Science and Technology, Guangxi University, Nanning, 530005, PR 
      China.
FAU - Zhou, Yanjun
AU  - Zhou Y
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China; Jiangsu 
      Co-innovation Center for Prevention and Control of Important Animal Infectious 
      Diseases and Zoonoses, Yangzhou, 225009, PR China.
FAU - Gao, Fei
AU  - Gao F
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China; Jiangsu 
      Co-innovation Center for Prevention and Control of Important Animal Infectious 
      Diseases and Zoonoses, Yangzhou, 225009, PR China.
FAU - Jiang, Yifeng
AU  - Jiang Y
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Yu, Lingxue
AU  - Yu L
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Zheng, Hao
AU  - Zheng H
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Tong, Wu
AU  - Tong W
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Yang, Shen
AU  - Yang S
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Zheng, Haihong
AU  - Zheng H
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Shan, Tongling
AU  - Shan T
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Liu, Fei
AU  - Liu F
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Xia, Tianqi
AU  - Xia T
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
FAU - Tong, Guangzhi
AU  - Tong G
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China; Jiangsu 
      Co-innovation Center for Prevention and Control of Important Animal Infectious 
      Diseases and Zoonoses, Yangzhou, 225009, PR China. Electronic address: 
      gztong@shvri.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140916
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Interferon Type I)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - *Gene Expression Regulation
MH  - Interferon Type I/biosynthesis/*immunology
MH  - MicroRNAs/genetics/*metabolism
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/*pathogenicity/*physiology
MH  - Real-Time Polymerase Chain Reaction
MH  - Viral Load
MH  - *Virus Replication
PMC - PMC7114497
OTO - NOTNLM
OT  - PRRSV
OT  - Type I interferon
OT  - Virus replication
OT  - miR-26a
EDAT- 2014/09/15 06:00
MHDA- 2015/07/24 06:00
PMCR- 2014/09/16
CRDT- 2014/09/15 06:00
PHST- 2014/07/10 00:00 [received]
PHST- 2014/08/12 00:00 [revised]
PHST- 2014/08/19 00:00 [accepted]
PHST- 2014/09/15 06:00 [entrez]
PHST- 2014/09/15 06:00 [pubmed]
PHST- 2015/07/24 06:00 [medline]
PHST- 2014/09/16 00:00 [pmc-release]
AID - S0168-1702(14)00340-2 [pii]
AID - 10.1016/j.virusres.2014.08.012 [doi]
PST - ppublish
SO  - Virus Res. 2015 Jan 2;195:86-94. doi: 10.1016/j.virusres.2014.08.012. Epub 2014 
      Sep 16.