PMID- 25219460
OWN - NLM
STAT- MEDLINE
DCOM- 20150803
LR  - 20231213
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 49
IP  - 6
DP  - 2014 Dec
TI  - Cryopreservation of ram semen in extenders containing soybean lecithin as 
      cryoprotectant and hyaluronic acid as antioxidant.
PG  - 934-40
LID - 10.1111/rda.12405 [doi]
AB  - A soybean lecithin-based extender supplemented with hyaluronic acid (HA) was 
      assayed for effectiveness to improve the quality of frozen-thawed ram semen. HA 
      has not been tested yet in an extender containing soybean lecithin for freezing 
      ram semen. Thus, the aim of this study was to analyse the effects of soybean 
      lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml(-1) in a Tris-based 
      extender on the motion characteristics, membrane integrity (HOST), viability, GSH 
      peroxidase (GSH-PX) activity, lipid peroxidation and acrosomal status after 
      freezing-thawing. Semen was collected from four Mehraban rams during the breeding 
      season and frozen in the six lecithinxHA extenders. The extender containing 1.5% 
      lecithin supplemented with no HA yielded higher total motility (52.5%+/-1.6), 
      viability (55.8%+/-1.6) and membrane integrity (44.5%+/-1.7), but the effects of the 
      lecithin concentration did not reach signification. Linearity-related parameters, 
      ALH, BCF, lipid peroxidation, GSH-PX activity, morphology and acrosomal status 
      were not affected by the extender composition. In general, adding HA 
      significantly decreased sperm velocity (1 mg ml(-1) HA), total motility (only 
      with 1.5% lecithin), viability (1 mg ml(-1) HA for 1% lecithin; both 
      concentrations for 1.5% lecithin) and membrane integrity. In conclusion, adding 
      HA to the freezing extender supplemented with soybean lecithin failed to improve 
      quality-related variables in ram semen. Increasing the lecithin content could 
      have a positive effect, but further studies are needed.
CI  - (c) 2014 Blackwell Verlag GmbH.
FAU - Najafi, A
AU  - Najafi A
AD  - Department of Animal Science, College of Agriculture, University of Tabriz, 
      Tabriz, Iran.
FAU - Najafi, M H
AU  - Najafi MH
FAU - Zanganeh, Z
AU  - Zanganeh Z
FAU - Sharafi, M
AU  - Sharafi M
FAU - Martinez-Pastor, F
AU  - Martinez-Pastor F
FAU - Adeldust, H
AU  - Adeldust H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140915
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (Antioxidants)
RN  - 0 (Cryoprotective Agents)
RN  - 0 (Lecithins)
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - Animals
MH  - Antioxidants/chemistry/pharmacology
MH  - Cryopreservation/*veterinary
MH  - Cryoprotective Agents/chemistry/pharmacology
MH  - Hyaluronic Acid/chemistry/*pharmacology
MH  - Lecithins/chemistry/*pharmacology
MH  - Male
MH  - Semen Preservation/*methods
MH  - Sheep/*physiology
MH  - Glycine max/*chemistry
EDAT- 2014/09/16 06:00
MHDA- 2015/08/04 06:00
CRDT- 2014/09/16 06:00
PHST- 2014/06/23 00:00 [received]
PHST- 2014/07/18 00:00 [accepted]
PHST- 2014/09/16 06:00 [entrez]
PHST- 2014/09/16 06:00 [pubmed]
PHST- 2015/08/04 06:00 [medline]
AID - 10.1111/rda.12405 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2014 Dec;49(6):934-40. doi: 10.1111/rda.12405. Epub 2014 Sep 
      15.