PMID- 25220289
OWN - NLM
STAT- MEDLINE
DCOM- 20150824
LR  - 20231213
IS  - 1879-1107 (Electronic)
IS  - 1096-4959 (Linking)
VI  - 177-178
DP  - 2014 Nov-Dec
TI  - Molecular characterization and functional divergence of two Gadd45g homologs in 
      sex determination in half-smooth tongue sole (Cynoglossus semilaevis).
PG  - 56-64
LID - S1096-4959(14)00123-7 [pii]
LID - 10.1016/j.cbpb.2014.09.001 [doi]
AB  - The growth arrest and DNA-damage-inducible protein 45 gamma (Gadd45g) is known to 
      play a major role in embryonic development and sex determination. In this study, 
      two Gadd45g genes were isolated from half-smooth tongue sole (Cynoglossus 
      semilaevis). Using chromosomal fluorescence in situ hybridization (FISH), 
      Gadd45g1 and Gadd45g2 were located on the W and Z chromosomes, respectively. The 
      full-length cDNA sequences of Gadd45g1 (1270bp) and Gadd45g2 (1181bp) were 
      predicted to contain a 480-bp coding sequence that could encode a protein of 159 
      amino acids residues. A phylogenetic tree showed that the predicted Gadd45g1 and 
      Gadd45g2 amino acid sequences clustered closely in one branch. It is proposed 
      that Gadd45g1 and Gadd45g2 are paralogous genes derived from the divergence of 
      the sex chromosome. Ka/Ks ratios indicated that Gadd45g1 and Gadd45g2 may have 
      undergone a high number of mutations and have a divergence time of only about 
      68,000years, although Gadd45g homologs are highly conserved. The qRT-PCR 
      demonstrated that Gadd45g1 and Gadd45g2 were highly expressed in ovary, and 
      negligibly expressed in testis of male and neo-male. During development of the 
      ovary (from 80 to 150days), the expression levels of both genes reached high 
      levels. Gadd45g1 was also highly expressed at 50days, the stage just before gonad 
      differentiation in C. semilaevis. All these findings imply functional divergence 
      of the two Gadd45g homologs; Gadd45g1 may be necessary for sex differentiation in 
      the early stage of gonad development, and then Gadd45g1 and Gadd45g2 maintain 
      ovary development and the female character of half-smooth tongue sole.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Liu, Wan-Jun
AU  - Liu WJ
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 
      Qingdao 266071, China; Fisheries College, Ocean University of China, 5 Yushan 
      Road, Qingdao 266003, China.
FAU - Zhang, Li-Yan
AU  - Zhang LY
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 
      Qingdao 266071, China.
FAU - Shao, Chang-Wei
AU  - Shao CW
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 
      Qingdao 266071, China.
FAU - Wang, Na
AU  - Wang N
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 
      Qingdao 266071, China.
FAU - Liu, Kun
AU  - Liu K
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 
      Qingdao 266071, China.
FAU - Wen, Hai-Shen
AU  - Wen HS
AD  - Fisheries College, Ocean University of China, 5 Yushan Road, Qingdao 266003, 
      China.
FAU - Zhang, Ning
AU  - Zhang N
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 
      Qingdao 266071, China.
FAU - Dong, Zhong-Dian
AU  - Dong ZD
AD  - Fisheries College, Ocean University of China, 5 Yushan Road, Qingdao 266003, 
      China.
FAU - Zhang, Jun-Jie
AU  - Zhang JJ
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 
      Qingdao 266071, China.
FAU - Chen, Song-Lin
AU  - Chen SL
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 
      Qingdao 266071, China. Electronic address: chensl@ysfri.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140908
PL  - England
TA  - Comp Biochem Physiol B Biochem Mol Biol
JT  - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
JID - 9516061
RN  - 0 (Intracellular Signaling Peptides and Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Evolution, Molecular
MH  - Female
MH  - Flatfishes/*metabolism
MH  - Intracellular Signaling Peptides and Proteins/genetics/*metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - Ovary/metabolism
MH  - Phylogeny
MH  - Sex Determination Processes
MH  - Sex Differentiation
MH  - Testis/metabolism
MH  - GADD45 Proteins
OTO - NOTNLM
OT  - Cynoglossus semilaevis
OT  - Gadd45g
OT  - Sex differentiation
EDAT- 2014/09/16 06:00
MHDA- 2015/08/25 06:00
CRDT- 2014/09/16 06:00
PHST- 2014/01/26 00:00 [received]
PHST- 2014/05/01 00:00 [revised]
PHST- 2014/09/01 00:00 [accepted]
PHST- 2014/09/16 06:00 [entrez]
PHST- 2014/09/16 06:00 [pubmed]
PHST- 2015/08/25 06:00 [medline]
AID - S1096-4959(14)00123-7 [pii]
AID - 10.1016/j.cbpb.2014.09.001 [doi]
PST - ppublish
SO  - Comp Biochem Physiol B Biochem Mol Biol. 2014 Nov-Dec;177-178:56-64. doi: 
      10.1016/j.cbpb.2014.09.001. Epub 2014 Sep 8.