PMID- 2522047
OWN - NLM
STAT- MEDLINE
DCOM- 19890417
LR  - 20181130
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 19
IP  - 1
DP  - 1989 Jan
TI  - Differential secretion of tumor necrosis factor-alpha and granulocyte/macrophage 
      colony-stimulating factors but not interferon-gamma from CD4+ compared to CD8+ 
      human T cell clones.
PG  - 197-200
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a pleiotropic lymphokine which may 
      have important regulatory effects on immune responses. It is shown here that 
      eight alloreactive CD4+ T cell clones (TCC) secreted significant amounts of 
      TNF-alpha after stimulation with either specific alloantigen or 
      12-O-tetradecanoylphorbol 13-acetate together with the calcium ionophore 
      ionomycin (up to 50 ng/ml/24 h/10(6) cells) whereas CD8+ TCC failed to do so 
      (max. 2 ng/ml/24 h/10(6) cells). The CD8+ TCC also secreted markedly less 
      granulocyte/macrophage colony-stimulating factor than the CD4+ cells. However, 
      this was not indicative of a general decrease of lymphokine production by CD8+ 
      cells because CD4+ and CD8+ TCC both secreted similar amounts of 
      interferon-gamma. These results show that regulatory CD4+ lymphocytes can produce 
      large amounts of TNF-alpha, whereas CD8+ effector cells cannot do so.
FAU - Pawelec, G
AU  - Pawelec G
AD  - Immunology Laboratory, Medizinische Klinik, Tubingen, FRG.
FAU - Schaudt, K
AU  - Schaudt K
FAU - Rehbein, A
AU  - Rehbein A
FAU - Busch, F W
AU  - Busch FW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Colony-Stimulating Factors)
RN  - 0 (Growth Substances)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Clone Cells/classification/immunology/metabolism
MH  - Colony-Stimulating Factors/*metabolism
MH  - Granulocyte-Macrophage Colony-Stimulating Factor
MH  - Growth Substances/*metabolism
MH  - Humans
MH  - Interferon-gamma/*metabolism
MH  - Lymphocyte Activation
MH  - Phenotype
MH  - T-Lymphocytes, Cytotoxic/immunology/metabolism
MH  - T-Lymphocytes, Helper-Inducer/classification/immunology/*metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
AID - 10.1002/eji.1830190132 [doi]
PST - ppublish
SO  - Eur J Immunol. 1989 Jan;19(1):197-200. doi: 10.1002/eji.1830190132.